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Investigation of Senile dementia of Alzheimer type(SDAT) in brain transplantation of rats.

大鼠脑移植中阿尔茨海默型老年痴呆（SDAT）的研究。

基本信息

批准号：
62480247
负责人：
IIZUKA Reiji
金额：
$ 3.01万
依托单位：
Juntendo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

Destruction and/or loss of cholinergic neurons in the nucleus basalis of Meynert are involved in Senile dementia of Alzheimer type(SDAT). The aim of the present study was to investigate the morphological and histochemical changes in cholinergic neurons and their circuitry in the aged rats and fetal brain tissues transplanted into the adult rats. In the first year, (1)in the immunohisto-chemical study of choline acetyltransferase(ChAT) using a monoclonal antibody, the ChAT-positive neurons in the ventral pallidum(VP) grafts showed morphological features identical to those in the host VP. (2)Electron microscopic observation revealed that neural circuits were well developed and formed synaptic contacts in the grafts. (3)Blood capillaries were developed in the junctional area between grafts and host tissue.In the second year, the morphology of ChAT-positive neurons of VP in aged rats(25 month-old) was compared to those in young adult rats(2 month-old). The size, the number of dendrites, the dendritic intersection and the length of dendrites of ChAT-positive neurons were significantly reduced in the aged group. These findings suggested that the rat VP cholinergic neurons were also affected by aging.In the final year, we modified acetylcholinesterase(AChE) histo chemical procedure to observe neuronal cell bodies and fibers in the transplants. Moreover, VP tissues transplanted in the lateral ventricle for one year will be compared to the transplants processed a short term.

Meynert基底核中胆碱能神经元的破坏和/或丢失参与阿尔茨海默型老年痴呆（SDAT）。本研究旨在观察老龄大鼠和胎脑组织移植入成年大鼠后胆碱能神经元及其回路的形态学和组织化学变化。在第一年，（1）胆碱乙酰转移酶（ChAT）单克隆抗体免疫化学研究中，腹侧苍白球（VP）移植物中ChAT阳性神经元的形态学特征与宿主VP相同。（2）电镜观察显示，移植物内神经回路发达，形成突触接触。（3）移植物与宿主组织交界处毛细血管发达，第2年比较老年大鼠（25月龄）和青年大鼠（2月龄）VP ChAT阳性神经元的形态。老年组ChAT阳性神经元的大小、树突数目、树突交叉点和树突长度均明显减少。最后一年，我们改良乙酰胆碱酯酶（AChE）组织化学方法，对移植物中的神经元胞体和纤维进行了观察。此外，将在侧脑室中移植一年的VP组织与短期处理的移植物进行比较。