Fundamental Studies to Elucidate Cerebral Developmental Impairment Mechanisms in Congenital Hydrocephalus and Development of Intrauterine Treatment.

阐明先天性脑积水脑发育障碍机制的基础研究和宫内治疗的发展。

• 批准号: 62480312
• 负责人: SATO Kiyoshi
• 金额: $ 2.24万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480312/
• 关键词: congenital hydrocephalus; congenital hydrocephalic rat; ventriculography; magnetic resonance imaging; ^<14>C-deoxyglucose; Golgi study; ventriculoperitoneal shunt; cerebral cortex; 脳室腹腔短絡術; 大脳皮質; ゴルジ法; シナプス; 螢光抗体法; 学習脳; 大脳皮貭; ゴルヂ法; 蛍光抗体法 /

We have experimentally treated the hydrocephalus of congenitally hydrocephalic HTX-rats by inserting a ventriculoperitoneal shunt (V-P shunt), at different times after birth, investigated through neuropathology and neurochemistry clarification of how timing of such surgery modifies impairment in development of neurocytes and synaptogenesis, and assessed the learning ability of the experimental animals.Experimental results obtained in 1987 suggested that placement of the V-P shunt delayed until around 4 weeks after birth of the hydrocephalic animals did not correct impairment of synaptogenesis of cortical pyramidal neurons and learning disability in comparison with non-hydrocephalic HTX-rats. In light of such observations, earlier placement of the V-P shunt at around 7 days after birth was carried out in experiments conducted in 1988 and 1989. The significance of the early placement was evaluated by various means, i.e., (1) chronological changes in lateral ventricle size and thickness o … More f the cortical mantle of the brain by means of Isovist ventriculography and magnetic resonance imaging(MRI), (2) measurement by 14C-deoxyglucose autoradiography of local cerebral glucose utilization (LCGU), (3) neuropathological techniques including rapid Golgi staining, (4) immunohistochemical assessment of synaptogenesis using monoclonal antibodies against a synaptic vesicle protein (SVP-38 of Obata et al., 1986), and (5) observation of learning ability by means of the Y-Arm-Maze Test.The results revealed that early placement of the V-P shunt was obviously effective in correcting reduced width of the cortical mantle and dilated ventricular size, mitigating cerebrospinal fluid white matter edema, normalizing the shape and arrangement of the ependymal cells lining the ventricular walls, preventing impairment of LCGU, restoring spine density of cerebral pyramidal neurons, and in decreasing learning disability. The present investigation would appear to have established a theoretical base supporting the necessity for early placement of a V-P shunt in animals with congenital hydrocephalus.Furthermore, the results may urge reconsideration of intrauterine treatment of congenital hydrocephalus in humans which a majority of neurosurgeons already have abandoned. Less

我们通过插入脑室腹腔分流术对先天性脑积水HTX大鼠的脑积水进行了实验性治疗（V-P分流术），通过神经病理学和神经化学研究阐明这种手术的时机如何改变神经细胞发育和突触发生的损伤，1987年获得的实验结果表明，V-与非脑积水HTX-大鼠相比，P分流延迟至出生后约4周，脑积水动物没有纠正皮质锥体神经元突触发生的损伤和学习障碍。根据这些观察结果，在1988年和1989年进行的实验中，在出生后7天左右较早放置V-P分流管。通过各种方法评估早期放置的意义，即，(1)侧脑室大小和厚度的时间变化 ...更多信息 通过Isovist脑室造影术和磁共振成像（MRI）测量大脑皮质套，（2）通过14 C-脱氧葡萄糖放射自显影术测量局部大脑葡萄糖利用（LCGU），（3）包括快速高尔基染色的神经病理学技术，（4）使用针对突触囊泡蛋白的单克隆抗体（SVP-38 of Obata et al.，结果表明，早期放置脑室-腹腔分流管对纠正脑皮质覆盖层宽度减小和脑室扩大，减轻脑脊液白色水肿，恢复室管膜细胞的形态和排列，防止LCGU受损，恢复大脑锥体神经元棘密度，减少学习障碍。目前的研究似乎已经建立了一个理论基础，支持在先天性脑积水动物中早期放置V-P分流管的必要性，此外，研究结果可能会促使重新考虑大多数神经外科医生已经放弃的人类先天性脑积水的宫内治疗。少

项目成果

Miyazawa T.et al: "A new method of quantitatively analyzing synapsis using a synaptic vesicle protein(SVP-38)monoclonalantibody." in preparation.

Miyazawa T.等人：“一种使用突触小泡蛋白（SVP-38）单克隆抗体定量分析突触的新方法。”

Miyaoka M.et al: "Measurement of local cerebral glucose utilization before and after VーP shunt in congenital hydrocephalus in rats." Metabolic Brain Disease. 3. 125-132 (1988)

Miyaoka M. 等人：“先天性脑积水大鼠 V-P 分流前后局部大脑葡萄糖利用的测量”，代谢性脑疾病。

佐藤潔 他: CLINICAL NEUROSCIENCE.

Kiyoshi Sato 等人：临床神经科学。

Miyaoka M. et al: "Measurement of local cerebral glucose utilization before and after V-P shunt in congenital hydrocephalus in rats." Metabolic Brain Disease 3, 125-132, 1988.

Miyaoka M. 等人：“先天性脑积水大鼠 V-P 分流前后局部脑葡萄糖利用率的测量”。

宮岡誠 他: "先天性水頭症ラットに対するVーPシャント前・後における脳局所グルコ-ス代謝の変化" 小児の脳神経. 13. 389-393 (1988)

Makoto Miyaoka 等人：“先天性脑积水大鼠 V-P 分流前后局部脑葡萄糖代谢的变化”《儿科神经病学》13. 389-393 (1988)。