Electron Microscopic Studies on Osteoclastic Bone Resorption

破骨细胞骨吸收的电子显微镜研究

• 批准号: 62480369
• 负责人: AMEMIYA Akira
• 金额: $ 0.64万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480369/
• 关键词: Bone Resorption; Osteoclast; Indomethacin; Alveolar Bone; Extraction Wound; 超微構造; 走査電顕; 透過電顕; プロスタグランジン; 微細構造

The present studies were intended to investigate the process of the osteoclastic bone resorption under several conditions and to clarify the structures and functions of osteoclasts and the mechanism of bone resorption. The results were summarized as follows:1. Comparative studies on osteoclastic bone resorption: The regions of bone resorption in rats were examined by histologic, scanning electron microscopic(SEM) and transmission electron microscopic(TEM) methods. The findings observed by SEM were consistent with the histological findings and those of TEM. It indicated that the comparative morphological investigation is one of the necessary and useful procedures.2. Effect of Indomethacin(IM) on osteoclastic bone resorptioin: In order to investigate the effect of IM on osteoclastic bone resorption in experimentally induced inflammatory lesion of periodontal tissue in rats, the resorption at the interdental bone induced by insertion of elastic band was examined histologically and ultrast … More ructurally. In the IM administrated rats, the reduction of the osteoclastic bone resorption was observed. It seems that the reduction of the bone resorbing activity is a consequence of the inhibition of prostaglandins (PGs) synthesis caused by IM. These results suggest that PGs play an important role on the osteoclastic bone resorption in inflammation of periodontal tissues.3. Osteoclastic bone resorption in the healing process of the extraction wound: In order to investigate the role of the bone resorption in the healing process of the extraction wound, the process in IM administrated rats was examined histologically and ultrastructurally. In the IM administrated rats, the retardation of healing process related to incomplete bone remodeling, which was caused by the inhibition of osteoclastic bone resorption and the reduction of new bone formation, was observed. These findings suggest that IM inhibits pgs synthesis and consequently reduces the activity of bone resorption and remodeling. Thus, it is considered that PGs as one of the chemical mediators perticipate also in the healing process of extraction wound and that osteoclasts, associated with osteoblasts, play an important role in this process. Less

本研究旨在探讨破骨细胞在不同条件下的骨吸收过程，阐明破骨细胞的结构和功能以及骨吸收的机制。主要研究结果如下：1.骨细胞骨吸收的比较研究：采用组织学、扫描电镜（SEM）和透射电镜（TEM）方法观察大鼠骨吸收的部位。扫描电镜观察结果与组织学和透射电镜观察结果一致。这表明，比较形态学研究是必要的和有用的步骤之一.吲哚美辛（IM）对大鼠牙周炎性病变骨吸收的影响：采用组织学和超微结构观察IM对牙周炎性病变大鼠牙间骨吸收的影响，并与对照组比较。 ...更多信息 闹哄哄的在IM给药大鼠中，观察到骨细胞骨吸收减少。骨吸收活性的降低似乎是IM抑制前列腺素（PGs）合成的结果。提示PGs在牙周组织炎症中对骨吸收起重要作用.拔牙创愈合过程中的骨吸收：为了研究骨吸收在拔牙创愈合过程中的作用，对IM给药大鼠的愈合过程进行了组织学和超微结构观察。在IM给药的大鼠中，观察到与不完全骨重建相关的愈合过程延迟，这是由骨细胞骨吸收抑制和新骨形成减少引起的。这些结果表明，IM抑制PGS合成，从而降低骨吸收和重塑的活动。因此，认为PGs作为化学介质之一也参与拔牙创的愈合过程，破骨细胞与成骨细胞在此过程中起重要作用。少

项目成果

Amemiya Akira: Recent Advances in Clinical Periodontology(Proceeding of the third Meeting of the International Academy. 555-560 (1988)

雨宫彰：临床牙周病学的最新进展（国际学会第三次会议记录。555-560（1988）

Koike, Kaoru: "Effect of Indomehacin on Healing Process of the Extraction Wound" Journal of Dental Research. 68. (1989)

小池薰：“吲哚美辛对拔牙伤口愈合过程的影响”牙科研究杂志。

Koike Kaoru: Journal of Dental Research. 68. (1989)

小池薰：牙科研究杂志。

Amemiya, Akira: "Effect of Indomethacin on Osteoclastic Resorption of Alveolar Bone" Recent Advances in Clinical Periodontology (Proceeding of the Third Meeting of the International Academy of Periodontology). 555-560 (1988)

Amemiya Akira：“吲哚美辛对牙槽骨破骨细胞吸收的影响”临床牙周病学最新进展（国际牙周病学会第三次会议论文集）。

Amemiya Akira: Recent Advances in Clinical Periodontology(Proceeding of the Third Meeting of the International Academy of Periodontology). 555-560 (1988)

雨宫明：临床牙周病学的最新进展（国际牙周病学会第三次会议记录）。