Relationship between Caries-detector Stainability and Ultrastructure of Carious Dentin

本研究旨在改进龋齿检测器，其具有较少的渗透性和渗透性进入外部龋坏牙本质，因此需要较长的治疗时间。用六种不同溶剂配制的酸性红溶液对人工脱钙牛牙本质和人龋坏牙本质进行体外渗透性测定。通过对人工脱钙牙本质的渗透性实验，考察了染料浓度和作用时间对渗透性的影响。因此，选择DMSO作为实验龋齿检测器溶液的合适溶剂。将10%柠檬酸添加到实验检测器中，以去除切割牙本质表面的涂抹层。因此，10%柠檬酸-1%酸性红-10%DMSO溶液被设计为一种新的龋检测器。通过体外和体内实验，与传统的探测器进行了比较，发现了以下事实：1。DMSO溶液对人工脱钙牙本质的渗透性最强.在丙二醇、乙二醇和DMSO溶液中，DMSO溶液对外层龋坏牙本质的渗透效果最好，说明DMSO溶液对人工脱钙牙本质有较好的渗透性. 1%酸性红丙二醇溶液对人工脱钙牙本质的渗透性随作用时间的延长而增强。高浓度的染料还使由上述溶剂制成的酸性红溶液对人工脱钙牙本质和龋坏牙本质的渗透性更大。而5%酸性红溶液的频繁使用则易使侧腔壁完整的牙本质表面着色.当在使用检测器溶液之前用稀柠檬酸去除涂抹层时，龋齿检测的频率显著降低。

This study was projected to improve the caries detector which had less stainability and permeability into the outer carious dentin and so required longer chair time for caries treatment. Permeability of acid red solutions made of six types of solvents were determined in vitro for an artificially decalcified bovine dentin and exracted human carious dentin. In addition, the effects of dye-concentration and applying time of these solutions were evaluated through the permeability test in the artificially decalcified dentin. As a result, DMSO was selected as a suitable solvent for an experimental caries detector solution. 10% citric acid was added to the experimental detector for removing the smeared layer on cut dentin surfaces. The 10% citric acid-1% acid red-10% DMSO solution was thus designed as a new caries detector. Efficiency of the new detector was also examined in vitro and in vivo compared with the conventional, finding the following facts;1. DMSO solution was the most permeable into the artificially decalcified dentin among six types of solutions.2. DMSO solution showed the most permeable effect in the outer carious dentin among propylene glycol, ethylene glycol and DMSO solution, which indicated relatively good permeability into the artificially decalcified dentin.3. The longer applying time exhibited great permeability of 1% acid red propylene glycol solution to the artificially decalcified dentin. High concentration of the dye also brought in greater permeability of the acid red solutions made of the above-mentioned solvents into both the artificially decalcified and the carious dentin. The frequent use of 5% acid red solution, however, was liable to stain the intact dentin surfaces of the side cavity walls.4. When the smeared layer was removed with thin citric acid prior to use of the detector soultion, frequency of caries detection remarkably decreased.