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Development of new indicator in biological malignancy of thyroid carcinoma preoperatively using aspiration biopsy cytology (ABC)

利用抽吸活检细胞学（ABC）开发甲状腺癌术前生物恶性程度的新指标

基本信息

批准号：
62480474
负责人：
MATSUYAMA Toshiya
金额：
$ 0.32万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

With the aim of preoperative determination of the biological malignancy of thyroid carcinoma, we first attempted immunological staining of cytologic specimens collected by aspiration biopsy. However this failed due to technical difficulties. Thus, we decided to attempt to determine the malignancy of thyroid carinoma by immunohistochemical examination of histologic specimens. Expression of C-myc p62, C-Ha-ras p21, epidermal growth factor (EGF), transforming growth factor (TGFalpha), and estrogen reception (ER), as indicators of malignancy, was observed/ The findings are summarized as follows:1. Immunological activity of C-myc p62 was expressed at a high frequency (91.7%) in undifferentiated carinomas having a poor prognosis. A particularly high ratio of patients with papillary carcinoma lymphnode metastasis were positive for the immunological activity (P<0.01).2. In carcinomas involving a larger number of Erpositive cells, lower values of EOPTC (an index of prognosis) were obtained, with a negative correlation of the ERpositivity ratio with prognosis.3. There was no definite correlation of the ratio of patients positive for c-Ha-ras p21, EGF or TGFalpha, with the histologic type or clinical malignacy of thyroid carinoma.4. Differential diagnosis of follicular adenoma and follicular carcinoma was impossible by an examination of the expression of these various indicators. to technical difficulties. Thus, we decided to attempt follicular carcinoma was impossible by an examination of Bases on these findings, it can be said that C-myc p62 abd ER in their expression are valuable as indicators of malignancy of thyroid carcinoma.It should also be noted that perfect preoperative differential diagnosis of medullary carcinoma and undifferentiated carcinoma was possible by comparing the expression of carcinoembrionic antigen and that of carcitonin on cytologic specimens collected by aspiration before surgery, i.e., our initial aim was successfully accomplished.

为了术前确定甲状腺癌的生物学恶性程度，我们首先尝试对抽吸活检收集的细胞学标本进行免疫染色。但由于技术上的困难而失败了。因此，我们决定尝试通过免疫组化检查的组织标本来确定甲状腺癌的恶性。观察C-myc p62、C-Ha-ras p21、表皮生长因子（EGF）、转化生长因子（TGF α）和雌激素受体（ER）的表达，作为恶性肿瘤的指标。C-myc p62免疫活性在预后差的未分化癌中表达率高（91.7%）。尤其是乳头状癌淋巴结转移患者的免疫活性阳性率更高（P<0.01）.在ER阳性细胞较多的癌中，EOPTC（预后指标）值较低，ER阳性率与复发呈负相关. c-Ha-rasp 21、EGF、TGF α阳性率与甲状腺癌的组织学类型、临床分期无明显相关性.滤泡性腺瘤和滤泡性癌的鉴别诊断是不可能的，通过检查这些不同的指标的表达。技术上的困难。因此，我们决定尝试滤泡癌是不可能的检查根据这些发现，可以说，C-myc、p62、ER的表达对判断甲状腺癌的恶性程度有一定的参考价值，同时应注意，通过比较细胞学上癌胚抗原和降钙素的表达，可对髓样癌和未分化癌进行准确的术前鉴别诊断手术前通过抽吸收集的样本，即，我们的初步目标成功地实现了。