权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

On the Studies of the Sterilized Egg Appearance and Their Metabolism in the Silkworm

家蚕无菌卵外观及其代谢的研究

基本信息

批准号：
63480047
负责人：
TAKEI Ryuzo
金额：
$ 3.97万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Appearance of sterilized eggs give much economical damage for seed production in the silkworm. Many studies have been carried out to resolve these problems and many points were declared. But much more issuestill remain to be done. So that author planed an following experiments to clear the mechanism for the appearance of sterilized eggs.1) Sterilized eggs strain was crossed with tri-molting strain and made a many genetical combinations. In following generation, sterilized eggs product batches were investigated. As a result, it is speculated that sterilized gene localize on the six chromosome as well as tri-molting gene loci.2) In order to investigate the relation to an appearance of sterilized eggs and egg components, amino acid, protein and fatty acid composition were investigated. There are some differences between sterilized and normal eggs in above substances. Sterilized eggs have much more than normal ones in isoleucine and aspartic acid and less in glutamic acid, alanine, methionine, lysine. Otherwise, in fatty acid composition, the former one has much less linolenic acid than later one.3) Developments of gonad and its spermatids injected juvenile hormone were investigated by cell sorter. It was declared that juvenile hormone inhibited a development of the gonad and spermatids and control the G_1 stage in cell division.From above results, I consider that appearance of sterilized eggs are controlled by the many genetical and non-genetical factors.

蚕种的出现给蚕种生产带来了很大的经济损失。为了解决这些问题，进行了许多研究，并提出了许多观点。但仍有许多问题有待解决。为此，作者进行了以下几个方面的研究：1）将不育系与三蜕皮系进行杂交，进行了多个遗传组合。在下一代中，对灭菌蛋产品批次进行了研究。结果表明，不育基因可能位于第六染色体上，同时也位于三蜕皮基因位点上。2）为了研究不育卵外观与卵成分的关系，对氨基酸、蛋白质和脂肪酸组成进行了研究。灭菌鸡蛋与正常鸡蛋在上述物质方面存在一定差异。灭菌鸡蛋中的异亮氨酸和天冬氨酸含量比正常鸡蛋高，而谷氨酸、丙氨酸、蛋氨酸、赖氨酸含量比正常鸡蛋低。另外，在脂肪酸组成中，前者的亚麻酸含量明显低于后者。3）用细胞分选仪观察了注射保幼激素后的性腺及其精子细胞的发育情况。保幼激素抑制性腺和精细胞的发育，控制细胞分裂的G_1期，认为不育卵的出现受多种遗传和非遗传因素的控制。