Neuronal Reguration of the Hypothalamic-hypophysial Axis Immunohistochemistry

下丘脑-垂体轴的神经元调节免疫组织化学

• 批准号: 63480096
• 负责人: DAIKOKU Shigeo
• 金额: $ 2.5万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480096/
• 关键词: Hypothalamus; Hypophysis; Neuronal regulation; Immunohistochemistry; Rat; 下垂体前葉支配; 神経機構; 下垂体前葉; 神経支配

We examined the neuronal regulation mechanisms of the hypothalamic-hypophysial system in rats. Our first aim was to find out synaptic associations between hypothalamic hypophysiotrophic hormone-containing neurons and neurons containing neuroactive substances which are known to affect the hypophysiotrophic hormone-containing neurons. Hence we developed a double immunolabeling technique to detect simultaneously different neuroactive substances in pre-and postsynaptic components in combining with intrahypothalamic surgery to transect neuronal pathways or with retrograde tracing of fiber tracts. Additionally we pursued the origins of hypothalamic hypophysiotrophic neurons by using organ culture and transplantation techniques of embryonal hypothalamic tissues. First of all we examined the origin of gonadotrophic hormone roleasing hormono (GaRH)-containing neurons. They derived from the nasal placode : migrating sequentially to the terminal ganglion and further to the internal surface of the … More telencephalon vesicles ; and reaching finally the septo-preoptic area, wherefrom, they extend axonal projections to the developing median eminence to release hormonal GnRH to the portal capiilaries. This suggests that secretory hypophysiotrophic GnRH neurons derive from sensory GnRH neurons. To know what this migration is induced by the nasal placode with the medial basal hypothalamus or cerebral cortex was cultured or transplanted into the third ventricle of adult rats. Secretory neurons with long axonal processes conjugated with blood capillaries to form median eminence like-structures appeared only in the grafts co-transplanted with the medial basal hypothalamus. GnRH neurons in the septo-prepotic area were synaptically regulated by substance P-containing neurons localized in the acurate nucleus. Further we have shown the development of hypothalamic neuropeptide Y-containing neurons and their synaptic associations with vasopressin-containing neurons in the paraventricular nucleus. Furthermore, we have demonstrated the teciprocal regultaory relations between somatostatin-containing neurons and corticotrophin releasing hormone-containing neurons in the parvocellular paraventricular nucleus. Less

我们研究了大鼠下丘脑-垂体系统的神经元调节机制。我们的第一个目标是找出下丘脑垂体促性腺激素神经元和神经元之间的突触协会含有神经活性物质，已知影响垂体促性腺激素神经元。因此，我们开发了一种双重免疫标记技术，结合下丘脑内手术切断神经元通路或逆行追踪纤维束，同时检测突触前和突触后成分中的不同神经活性物质。此外，我们还利用胚胎下丘脑组织的器官培养和移植技术，探讨了下丘脑垂体营养神经元的起源。首先，我们研究了促性腺激素释放激素（GaRH）的神经元的起源。它们起源于鼻基板：依次迁移至终神经节，并进一步迁移至鼻神经节的内表面。 ...更多信息 端脑小泡;最后到达隔-视前区，从那里，它们将轴突投射延伸到发育中的正中隆起，以向门静脉毛细血管释放激素GnRH。这表明分泌型促垂体GnRH神经元来源于感觉型GnRH神经元。为了了解这种迁移是由下丘脑或大脑皮质内侧基底部的鼻基板培养或移植到成年大鼠的第三脑室中引起的。长轴突突起的分泌神经元与毛细血管结合形成正中隆起样结构，仅出现在与内侧基底下丘脑共移植的移植物中。隔-肛区的GnRH神经元受位于尖核的P物质神经元的突触调节。此外，我们已经显示了下丘脑神经肽Y的神经元的发展和他们的突触协会与加压素的神经元在室旁核。此外，我们还证实了小细胞室旁核内含生长抑素神经元和含促肾上腺皮质激素释放激素神经元之间的相互调节关系。少

项目成果

Kagotani,Y.,Hisano,S.,Tsuruo,Y.,Daikoku,S.,Chihara,K.: "Vasopressinーdeficient paraventricular magnocellulal neurons of homozygous Brattleboro rats synthesize neuropeptide Y." Neuroscience Letters. 112. 37-42 (1990)

Kagotani, Y.、Hisano, S.、Tsuruo, Y.、Daikoku, S.、Chihara, K.：“纯合子 Brattleboro 大鼠缺乏加压素的室旁大细胞神经元合成神经肽 Y。” 112. 37-42（《神经科学快报》） 1990）

Yokote, R., Hisano, S., Daikoku, S.,: "Immunohistochemical localization of glucocorticoid receptors in anterior pituitary cells of rats." Arch. Histol. Cytol.(1991)

Yokote, R.、Hisano, S.、Daikoku, S.：“大鼠垂体前叶细胞中糖皮质激素受体的免疫组织化学定位。”

Hisano,S.,Tsuruo,Y.,Kagotani,Y.,Daikoku,S.Chihara,K.: "Immunohistochemical evidence for synaptic connections between neuropeptide Yーcontaining axons and periventricular somatostatin neurons in the anterior hypothalamus in rats." Brain Res.520. 170-177 (19

Hisano, S.、Tsuruo, Y.、Kagotani, Y.、Daikoku, S. Chihara, K.：“大鼠下丘脑前部含神经肽 Y 的轴突与室周生长抑素神经元之间突触连接的免疫组织化学证据。” .520。170-177（19

Kawado,H.,Tsuruko,Y.,Bando,H.,Daikoku,S.: "Hypothalamic TRHーproducing neurons identified by combining immunohistochemistry for proーTRH and retrograde tracing." J.Comparative Neurology. (1991)

Kawado, H.、Tsuruko, Y.、Bando, H.、Daikoku, S.：“通过结合免疫组织化学进行 TRH 和逆行追踪来鉴定产生 TRH 的神经元”（J. 比较神经学）。

Daikoku,S.,Tsuruo,Y.,Hashimoto,T.,Okamura,Y.,Yokote,R.: "Hypothalamic neurons from a developmental aspect." Arch.Histol.Cytol.52,Suppl.217-223 (1989)

Daikoku，S.，Tsuruo，Y.，Hashimoto，T.，Okamura，Y.，Yokote，R.：“从发育角度看下丘脑神经元。”