Molecular mechanism of the abnormal expression of MHC-linked complement genes.

MHC相关补体基因异常表达的分子机制。

• 批准号: 63480167
• 负责人: TAKAHASHI Morinobu
• 金额: $ 4.29万
• 依托单位: Cancer Research Institute, Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480167/
• 关键词: Transcriptional regulation; Complement C4; Slp gene; Complement deficiency; Transciptional factor; トランススペライシング; トランススプライシング; 補体遺伝子; CATアツセイ; mRNAの安定性

Regulatory mechanism underlying the abnormal expression of complement C4 and Slp genes of mouse was investigated at molecular level, using a wide variety of techniques of molecular biology. A few very important findings have been obtained from the present work which are summarized as follows.I.Post transckiptional and pretranslational regulation was shown to determine low C4 expression of C4 gene of H-2^k mouse(BlO.BR). C4 cDNA was isolated from the liver cDNA library which contains sequences probably derived by transsplicing mechanism.The transcriptional regulatory domains were identified in the 5' region of mouse C4 and Slp genes. The recognition mothif homologous to NF.kB/H2TF^1_ was identified in the region of C4 gene but not of Slp gene. Nuclear factors of mouse liver binds to this recognition mothif of C4 gene and probably enhance the transciption of C4 gene.

本研究运用多种分子生物学技术，从分子水平探讨了小鼠补体C4和Slp基因异常表达的调控机制。本文的研究结果如下：1. H-2^k小鼠C_4基因的低表达受转录后和翻译前的调控。从小鼠肝脏cDNA文库中分离到C4基因cDNA，并在C4和Slp基因5'端区域鉴定了转录调控结构域。与NF.kB/H2TF^1_同源的识别方式在C4基因区被鉴定，而在Slp基因区未被鉴定。小鼠肝细胞核因子与C4基因的这种识别方式结合，可能增强C4基因的转录。

项目成果

Yu, D. Y., Huang, Z.M., Murakami, S., Takahashi, M., & Nonaka, M: "Specific binding of a hepatoma nuclear factor to the NF. kappaB/H2TF1 recognition motif found in the C4 promoter, but not in the Slp promoter." Journal of Immunology 143, 2395-2400, 1989.

余大义、黄志明、村上S、高桥M、

Sa-nga,P.,Nakayama,K.,Nonaka,M.,& Takahashi,M.: "Occurrence of aberranthy spliced mRIVA associated with Complement C4 gene of low C4 producing mouse" Proc.Natl.Acad.sci,USA.

桑加，P.，中山，K.，野中，M.，

Yu,D.Y.,Huang,Z.M.,Murakami,S.,Takahashi,M.& Nonaka,M.: "Specific binding of a hepatoma nuclear factor to the NF.kB/H2TF1 recognition motif found in the C4 promoter,but not in the SLp promoter." Journal of Immunology. 143. 2395-2400 (1989)

余大义、黄ZM、村上S、高桥M

Yu, D. Y., Nonaka, M. & Takahashi, M: "Mapping of the transcriptional regulatory domains for the difference in the promoter activity between mouse C4 and Slp(sex limited protein) genes." Journal of Immunology 141, 4381-4387, 1988.

Yu, D. Y., 野中 M.

Nakayama,K.,Pattanakitsakul,S.,Yokoyama,S.……Takahashi,M.: "Posttranscriptional regnlation of complement C4 in low C4 producing strain of mouse." Immunogenetics(印刷中). (1990)

Nakayama, K.、Pattanakitsakul, S.、Yokoyama, S....Takahashi, M.：“低 C4 产生小鼠品系中补体 C4 的转录后调节”（出版中）。