Studies on Mechanism of Proliferation of Lymphocytis to Produce Platelet Associated Immunoglobulin.

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1) Mechanism of proliferation of PAIgG-secreting lymphocytes.We developed the new modified methods of ELIZA-plaque assay which could identify and count the lymphocytes to secrete platelet associated IgG (PAIgG) in mouse spleen cells sensitized with human platelets. The proliferation of PAIgG secreting spleen cells of mouse were suppressed with such drugs as cyclophosphamide, bolus prednisolone and prednisolone of long period. However, we could not detect PAIgG-secreting lymphocytes in spleen cells of children with chronic ITP and the peripheral lymphocytes of the patient sensitized with isologous platelets transfusion.On the other hand, we immunized mouse with IgG fraction of the children whose sera contained platelet bound IgG and produced the polyclonal antibody for antihuman IgG. Using this antibody, we could found incresed number of the lymphocytes combined with IgG (LAIgG) in the peripheral blood of children with chronic ITP, Evans syndrome and ulcerative colitis. Then, the lymphocytes with LAIgG were shown to be T-cells. The student are going to make clear the significance of the increase of lymphocytes with LAIgG in those children.2) Detection of antigen site on the palatelet membrane combined with autoantibody.We measured the amount of monoclonal antibodies to conbine with platelet membrane as for platelets of children with chronic ITP, Evans syndrome and thromboasthenia. Five of 22 children with chronic ITP showed decreased amount of monoclonal antibodies combined with platelet membrane. This phenomenon suggested that there were the antigen site to combine with autoimmune platelet antibodies near the epitope of GPIIb/IIIa.