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MALIGNANT POTENTIALITY OF LIVER CIRRHOSIS AND HEPATOCELLULAR CARCINOMA FOR FACTORS INFLUENCING THE PROGNOSIS -ANALYSIS OF DNA DISTRIBUTION AND CELL KINETICS -

肝硬化和肝细胞癌的恶性潜能影响预后的因素-DNA分布和细胞动力学分析-

基本信息

批准号：
63480284
负责人：
KUSANO Mituo
金额：
$ 1.98万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

The evaluation of the cell kinetics of liver cirrhosis and liver cancer may lead to the appropriate therapeutique means and prediction of preneoplastic state of liver cirrhosis. Not only DNA analysis by flow cytometry(FCM) but also parameters of cell kinetics have been playing an increasingly significant role in the biology and immunology as well as in the clinical oncology, and it may be preferred method for the assessment of the biological characteristics of liver cells. But, no flow cytometric study on human liver cells has been done due to the difficulties in the preparation of single liver cell suspensions from clinical materials.In the experiment with rats, proliferating activities of cirrhotic and cancerous liver cells were assessed by DNA-synthesizing S phase after pulse-labeling monoclonalantibody to BrdU.The relative DNA content, the incidence of aneuploidy and their clinical implications were examined on 102 specimens of liver cancers and their surrounding liver cirrhosis. In a total of 50 HCCS, 16 demonstrated the DNA diploidy and the remaining 34(68%) showed the DNA aneuploidy. It showed a tendency toward the better prognosis in the group of the DNA diploidy than that of the DNA aneuploidy group.In the experimental studies, both DNA ploidy pattern and parameters of cell kinetics were useful for the assessment of the proliferation activities of the liver cells, but no noted relation between these parameters and their malignant potentiality was found.The analysis of nuclear DNA content and cell kinetics of the liver cells may offer good indicator for the evaluation of the malignant potentiality of liver cancer. Furthermore, it may enable us to predict the risk of the malignant transformation of liver cirrhosis which is considered as a preneoplastic condition.

评价肝硬化和肝癌的细胞动力学，可为肝硬化的治疗提供依据，并可预测肝癌前状态。流式细胞术（FCM）DNA分析和细胞动力学参数在生物学、免疫学和临床肿瘤学中发挥着越来越重要的作用，有望成为评价肝细胞生物学特性的首选方法。本实验用BrdU单克隆抗体脉冲标记大鼠肝细胞DNA合成S期，测定肝细胞DNA相对含量，用流式细胞术检测肝细胞增殖活性，结果表明，肝细胞DNA相对含量为100%~ 200%。本文对102例肝癌及癌旁肝硬化标本进行了非整倍体发生率及其临床意义的研究。在50例HCCS中，16例显示DNA二倍体，其余34例（68%）显示DNA非整倍体。在实验研究中，DNA倍体类型和细胞动力学参数对评价肝细胞增殖活性是有用的，肝细胞核DNA含量和细胞动力学分析表明，可为肝癌恶性潜能的评价提供良好的指标。此外，它可能使我们能够预测被认为是癌前病变的肝硬化恶变的风险。