MALIGNANT POTENTIALITY OF LIVER CIRRHOSIS AND HEPATOCELLULAR CARCINOMA FOR FACTORS INFLUENCING THE PROGNOSIS -ANALYSIS OF DNA DISTRIBUTION AND CELL KINETICS -

对肝硬化和肝癌的细胞动力学进行评估，可以指导适当的治疗方法和预测肝硬化的癌前状态。流式细胞术分析DNA和细胞动力学参数不仅在生物学、免疫学和临床肿瘤学中发挥着越来越重要的作用，而且可能成为评价肝细胞生物学特性的首选方法。但是，由于临床材料制备单个肝细胞悬浮液的困难，尚未对人肝细胞进行流式细胞术研究。在大鼠实验中，通过脉冲标记BrdU单克隆抗体后的dna合成S期评估肝硬化和癌变肝细胞的增殖活性。本文检测了102例肝癌及其周围肝硬化患者的相对DNA含量、非整倍体发生率及其临床意义。在50例HCCS中，16例显示DNA二倍体，其余34例（68%）显示DNA非整倍体。DNA二倍体组预后优于DNA非整倍体组。在实验研究中，DNA倍体模式和细胞动力学参数对评估肝细胞的增殖活性都是有用的，但这些参数与肝细胞的恶性潜能之间没有明显的关系。肝细胞核DNA含量和细胞动力学分析可为评价肝癌的恶性潜能提供良好的指标。此外，它可以使我们预测肝硬化恶性转化的风险，肝硬化被认为是一种肿瘤前状态。

The evaluation of the cell kinetics of liver cirrhosis and liver cancer may lead to the appropriate therapeutique means and prediction of preneoplastic state of liver cirrhosis. Not only DNA analysis by flow cytometry(FCM) but also parameters of cell kinetics have been playing an increasingly significant role in the biology and immunology as well as in the clinical oncology, and it may be preferred method for the assessment of the biological characteristics of liver cells. But, no flow cytometric study on human liver cells has been done due to the difficulties in the preparation of single liver cell suspensions from clinical materials.In the experiment with rats, proliferating activities of cirrhotic and cancerous liver cells were assessed by DNA-synthesizing S phase after pulse-labeling monoclonalantibody to BrdU.The relative DNA content, the incidence of aneuploidy and their clinical implications were examined on 102 specimens of liver cancers and their surrounding liver cirrhosis. In a total of 50 HCCS, 16 demonstrated the DNA diploidy and the remaining 34(68%) showed the DNA aneuploidy. It showed a tendency toward the better prognosis in the group of the DNA diploidy than that of the DNA aneuploidy group.In the experimental studies, both DNA ploidy pattern and parameters of cell kinetics were useful for the assessment of the proliferation activities of the liver cells, but no noted relation between these parameters and their malignant potentiality was found.The analysis of nuclear DNA content and cell kinetics of the liver cells may offer good indicator for the evaluation of the malignant potentiality of liver cancer. Furthermore, it may enable us to predict the risk of the malignant transformation of liver cirrhosis which is considered as a preneoplastic condition.