Bioscientific study of the therapy for lung small cell cancer

1.抗癌药物敏感性（1）手术标本癌细胞的制备：将切碎的肺癌组织通过筛网过滤，并通过Ficoll Paque分离。该方法存在的问题是：（1）残留在筛网上的癌细胞数量不可忽略;（2）在分离步骤中，发生淋巴细胞和成纤维细胞的污染。酶法（克拉霉素酶、脱氧核糖核酸酶、胰蛋白酶）存在如下问题：（1）酶处理后粘度增加，过滤相当困难，处理后癌细胞生长速度下降。（2）培养基：在10%FCS条件下，RPM 11640、HA M、IMDM、MEM四种培养基的细胞生长率无差异。在这些培养基中，成纤维细胞显著增殖而不是癌细胞。无血清培养液、HITES（SCLC）、SCL-4（adeno）对成纤维细胞增殖有抑制作用，癌细胞存活时间短于有血清培养液。2-l 关于我们 艾耶尔琼脂糖培养基中，控制成纤维细胞增殖，而检测的癌细胞不增殖。这表明，用这些培养基不能在一周内进行抗癌药物的敏感性试验，而72小时内的实验体系或另一种评价癌细胞活性及其分子水平的方法值得研究.肺癌患者淋巴细胞Tac、KOLT-2、YTA-1抗原表达的免疫反应，但手术对其影响不大，转移（+）淋巴结淋巴细胞KOLT-2抗原表达较转移（-）淋巴结淋巴细胞增强。对IL-2或YTA-1的增殖反应有待研究。在许多恶性肿瘤中，leu 7的表达随病变的切除而增强，而良性肿瘤手术前后无明显变化。探讨其与手术根治性、双色流式细胞术及NK活性等的关系。少

1. Sensitivity for anticancer drug (1)preparation of cancer cell from surgical specimen: Minced lung cancer tissue was filtrated though mesh, and separated by Ficoll Paque. The problems of this method were (1) the number of cancer cells whitc h remained on the mesh is not negligible, (2) at the step of separation, the contamination of lymphocyte s and fibroblast cells occurred. The enzyme(cllagenase, Dnase, trypsin) method had the Problems such as (1) viscosity was increased after enzyme treatment, making filtration rather difficult, 0 the growth rate of cancer cells decreased after this treatment.(2)culture medium: With 10% FCS, between RPM11640, HA M, IMDM, and MEM, there is no difference about cell growth rate. In these mediums, fibroblast cells proliferated significantly rather than cancer cells. With serum-free medium, HITES(SCLC), SCL-4(adeno), however, the proliferation of fibloblast cell was suppressed, survival period of cancer cell was shorter than that with serum. With two-l … More ayer agarose medium, fibloblast cells were controlled to proliferate, and examined cancer cells were not proliferated. This suggest that with these medium, sensitivity test of anti-cancer drugs can not be done over one week period, and experimental system within 72 hours or the another evaluation method of the activity of cancer cell and its molecular level are thought to be studied.2. Immunological response of the host with lung cancer To the expression of Tac, KOLT-2, YTA-1 antigens on lymphocyte, however, the effect of operation was not recognized,the expression of KOLT-2 antigen was augmented on the lymphocytes of metastasis(+) lymphonode in comparison with that of metastasis(-) lymphonode in some cases. The prolifalative response to IL-2 or YTA-1 is to be studied. In many malignant cases, the expression of leu7 was augmented with resection of its lesion, there was no remarkable change on operation in benign cases. The relation with operative radicality, two color FACS with leull, NK activity, and so on are to be examined. Less