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Pathophysiology of multiple organ failure

多器官衰竭的病理生理学

基本信息

批准号：
63480353
负责人：
OKADA Kazuo
金额：
$ 3.97万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Severe insult to the body caused the cellular damage,which might contribute to establish the multiple organ failure.However,the changes in oxygen metabolism in such a condition were not well studied,so we tried to measure in situ cytochrome oxidase in different conditions,applying near infra-red spectrometric method.Near infra-red spectrometer was newly designed to improve the maneuver such as the innovation of the non-invasive sensor to brain or liver,suitable absorption wave lengths were determined to be able to separate hemoglobin and cytochrome oxidase absorption.Rats were anesthetized and blood was exchanged with fluosol which could transports the dissolved oxygen more effectively than the plasma itself. Blood being exchanged with fluosol,it is possible to neglect the influence of hemoglobin absorption upon cytochrome absorption. After then,inspired oxygen was gradually decreased about 10%,whereas the artificial respiration,was maintained at the same condition. As long as the oxygen concentration remained low, cytochrome oxidase was continued to be in a reduced state. The cellular oxygen. environment was shown to be severely deranged.On the other series, blood was exsanguidated to the level at which 50mmHg of systolic blood pressure was maintained. In this condition cytochome oxidase was rather well maintained at normal level compared to that in hypoxic series. Measurement of cytochrome oxidase would be a useful parameter to analyze the cellular derangement in the multiple organ failure.

严重的身体损伤导致细胞损伤，可能导致多器官功能衰竭。然而，在这种条件下氧代谢的变化并没有得到很好的研究，所以我们尝试在不同条件下原位测量细胞色素氧化酶，采用近红外光谱法。新设计了近红外光谱仪，改进了对大脑或肝脏的无创传感器的操作，确定了合适的吸收波长，可以分离血红蛋白和细胞色素氧化酶的吸收。麻醉大鼠后，用比血浆更有效地输送溶解氧的氟醇交换血液。血液与氟醇交换时，有可能忽略血红蛋白吸收对细胞色素吸收的影响。此后，吸入氧逐渐减少约10%，而人工呼吸维持在相同条件下。只要氧浓度保持在较低水平，细胞色素氧化酶就继续处于还原状态。细胞中的氧。环境显示出严重的紊乱。在另一组中，将血液放血至维持收缩压50mmHg的水平。在这种情况下，细胞色素氧化酶较低氧组维持在正常水平。细胞色素氧化酶的测定是分析多脏器功能衰竭时细胞紊乱的一个有用参数。