Liquid handling automated workstation for cell based assays

用于细胞检测的液体处理自动化工作站

• 批准号: 439328175
• 金额: --
• 依托单位: Julius-Maximilians-Universität Würzburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/439328175?language=en
• 关键词: Liquid; handling; automated; workstation; cell

We are a team of five research groups working on a wide range of topics at the interface between Biology and Medicine, specifically on cell and molecular biology of bacterial pathogens, antimicrobials and antifungals, host-pathogen interactions and cancer biology. While working on largely diverse topics, we share a common goal of applying innovative high-throughput approaches to tackle our research questions. In order to do so, we hereby apply for financial support for establishing a cutting-edge liquid handling automated workstation. With this instrumentation specially designed to develop novel high-throughput cell based assays, we propose to:• Perform a large-scale assessment the bactericidal effect of drug combinations against gram-negative bacterial pathogens, therefore providing new insights for fighting the alarming rise of antibiotic resistance. For a handful of potent synergistic combinations, we propose to re-assess their intracellular efficacy against persistent gram-negative pathogens, which are increasingly acknowledged to cause relapsing infections. The underlying mechanisms of bacterial cell death will be investigated using bacterial mutant libraries and conventional molecular biology techniques.• Explore large compound libraries to systematically investigate i) particular stress-response mechanisms in gastrointestinal bacterial pathogens – efflux pumps, small RNA regulators, small proteins – and how they relate to drug resistance and interaction with the human host; ii) how the host metabolism rewires in response to infection by intracellular pathogens which cause sexually transmitted diseases; iii) potential inhibitors of cancer specific protein-protein interactions.• Establish a large and well-characterised Candida spp. isolate collection, therefore elucidating the current antifungal resistance and epidemiological situation of one of the most prevalent life-threatening fungal pathogens in Germany.The automated workstation – to which we currently have no access – is instrumental to accomplish the described projects, and it would therefore tremendously support our research.

我们是一个由五个研究小组组成的小组，在生物学和医学之间的界面上，特别是在细菌病原体，抗菌和抗真菌剂，宿主 - 病原体相互作用和癌症生物学方面的细胞和分子生物学方面的广泛主题。在研究大型潜水员主题的同时，我们有一个共同的目标，即采用创新的高通量方法来解决我们的研究问题。为了这样做，我们特此申请财务支持，以建立尖端的液体处理自动化工作站。通过专门设计旨在开发新型高通量细胞评估的仪器，我们建议：•进行大规模评估，药物组合对革兰氏阴性细菌病原体的细菌作用，因此为抗击抗生素耐药性的令人震惊的新见解提供了新的见解。对于少数潜在的协同组合，我们建议将其细胞内效率重新评估针对持续的革兰氏阴性病原体，这些病原体越来越多地承认会引起复发感染。细菌细胞死亡的潜在机制将使用细菌突变库和常规分子生物学技术研究。•探索大型化合物库以系统地研究i）胃肠道细菌病原体中特定的应激 - 响应机制 - 外喷泵 - 外喷泵 - 小型RNA调节剂，小rna蛋白质，与人类的抗药性 - 与它们相互作用 - 与它们相互作用，它们相互作用及其相互作用，它们的相互作用; ii）宿主代谢如何响应细胞内病原体感染而导致性传播疾病的感染； iii）癌症特异性蛋白质蛋白相互作用的潜在抑制剂。孤立收集，因此阐明了德国最普遍的威胁生命真菌病原体之一的当前抗真菌抗药性和流行病学状况。自动化工作站（我们目前无法访问）对完成所描述的项目至关重要，因此将极大地支持我们的研究。