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Biological Function of Retinoic Acid Receptors in Bronchopulmonary System

支气管肺系统视黄酸受体的生物学功能

基本信息

批准号：
03807046
负责人：
AKITA Hirotoshi
金额：
$ 1.22万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807046/
关键词：
Lung Cancer Retinoic Acid Retinoic Acid Receptor レチノイン酸リセプタ-

项目摘要

Purpose: In this study, the expression of retinoic acid receptor(RAR) alpha and beta was examined in order to understand the role of retinoic acid(RA) in the development and function of bronchopulmonary system.Result: RARalpha was shown to be expressed in human adult lung, and rat adult and newborn lung in Northern blot analysis. This data suggested the important role of RA and RARalpha in the development and function of bronchopulmonary system. On the other hand, the expression of RARbeta gene was not detected in human adult lung, and rat newborn and adult lung in Northern blot analysis. This data suggested the tissue-soecific expression of RARbeta gene. Expression and structural abnormality of RARbeta gene was studied in human lung cancer cell lines using RNAse protection assay, since RARbeta gene was known to be located in the short arm of chromosome 3. No expression of RARbeta gene was detected in human lung cancer cell lines. This finding suggested that the loss of the RARbeta expression could be a very important feature in the process of carcinogenesis and progression of human lung cancer.In the future, it is important show the possibility of the clinical application of the RA-RAR system by studying whether it is possible to inhibit the transformation of cells with RA and/or RAR, and whether it is possible to reverse the transformed phenotype of cancer cells with RA and/or RAR.

目的：本研究采用北方杂交技术检测了人肺、大鼠肺和新生大鼠肺组织中视黄酸受体（RA）α和β的表达，以探讨RA在支气管肺系统发育和功能中的作用。提示RA和RAR α在支气管肺系统的发育和功能中起重要作用。另一方面，在北方印迹分析中，RAR β基因在成人肺、新生大鼠和成人肺中未检测到表达。提示RAR β基因表达具有组织特异性。由于已知RAR β基因位于3号染色体的短臂上，因此使用RNA酶保护试验研究了RAR β基因在人肺癌细胞系中的表达和结构异常。在人肺癌细胞系中未检测到RAR β基因的表达。这一发现表明，RAR β表达的缺失可能是人类肺癌发生和发展过程中的一个非常重要的特征。未来，重要的是通过研究是否可能抑制RA和/或RAR的细胞转化来显示RA-RAR系统临床应用的可能性。以及是否有可能逆转具有RA和/或RAR的癌细胞的转化表型。