Study of lymphocyte growth activity expressed on oligodendroglial membrane

点击翻译按钮获取中文摘要

We analyzed cellular interactions between T lymphocytes and a recently established immortalized glial line, L3 that retains several properties of immature oligodendrocytes (Aloisi et al., J Neurosci Res 27 : 16-24, 1990). L3 oligodendrocytes (L3-OL) cannot be induced to express class II antigens, nor do they specifically present antigen to syngeneic specific T lymphocyte. However, L3-OL strongly enhance the proliferation of freshly activated, interleukin-2 (IL-2)-dependent T line lymphocytes and concanavalin A (ConA)-activated lymphoblasts, irrespective of their antigen specificity of surface phenotype (CD4+ or CD8+). The mitogen-ic effect of L3-OL was observed in paraformaldehyde-fixed L3-OL cells and in membrane preparations, but not in culture supernatant. Neither intact L3-OL cells nor membrane preparations had direct IL-2 activity. The conclusion that the mitogenic effect of L3-OL cells is exerted by membrane structures acting as a costimulatory factor(s) of IL-2 is supported by the finding that it is largely blocked by a monoclonal anti-IL-2 receptor antibody. The effect is distinct from membrane-bound IL-1, membrane-bound tumor necrosis factor-alpha, IL-3, or IL-6 and cannot be reconstituted by these cytokines.