The Role of Immure Cells in Liver Metastasis of Paucreatic Cariroma

免疫细胞在少发性癌肝转移中的作用

• 批准号: 03807082
• 负责人: KOBARI Masao
• 金额: $ 1.02万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03807082/
• 关键词: Pancreatic Cancer; Adoptite Immunorlorapy; LAK; Liver Metastasis; Basement Membrare Proteirs; NK; Kupffer細飽; Kupffer細胞; LAK細胞; 膵癌肝転移

The killing activities of LAK cells obtained from pancreatic cancer patients were 77.9% for NK sensitive cells, 72.9% for LAK sensitive cells, and 36.7%, 23.2%, 86.4% for cultured human pancreatic cancer cell lines PK-1, PK-9, and KLM-1 respectively. The growth of KLM-1 cells inoculated on nude mice was inhibited by LAK cells in vivo. LAK cells with high killing activity against pancreatic cancer cells can be obtained from patients with pancreatic cancer. Laminin and fibronection on the surface of hepatocytes can mediate pancreatic cancer cells attachment and migration in the early phase of liver metastasis. The liver metastasis model formed by a highly metastatic variant (KLM-1) is valuable for the study of the liver metastatic processes of human pancreatic cancer.

胰腺癌患者LAK细胞对NK敏感细胞的杀伤活性为77.9%，对LAK敏感细胞的杀伤活性为72.9%，对培养的人胰腺癌细胞系PK-1、PK-9和KLM-1的杀伤活性分别为36.7%、23.2%和86.4%。LAK细胞在体内可抑制裸鼠KLM-1细胞的生长。从胰腺癌患者体内可获得对胰腺癌细胞具有高杀伤活性的LAK细胞。肝细胞表面的层粘连蛋白和纤维连接可介导胰腺癌细胞在肝转移早期的附着和迁移。由高转移变异体（KLM-1）形成的肝转移模型对于研究人类胰腺癌的肝转移过程具有重要价值。

项目成果

Y.Kimura: "Establishment of an experimental live metastasis model by intraportal injection of a nowly derived human pancreatic cancer cell live(KLM-1)." International Journal of Pancreatology. 20. 43-50 (1996)

Y.Kimura：“通过门静脉内注射现已衍生的活人胰腺癌细胞 (KLM-1) 建立实验性活体转移模型。”

Amikura K,Kobari M,and Matsuno S: "The mechanism of livermetastasis in caranoma of the pancreas-the role of laminir, fibronedin, type IV collagen in the attachment and migrotion of human pancreatic cancer cells." Japanese Journal of Pancreatology. 11. 205

Amikura K、Kobari M 和 Matsuno S：“胰腺癌肝转移的机制——laminir、纤连蛋白、IV 型胶原在人胰腺癌细胞附着和迁移中的作用”。

遊佐 透: "膵癌患者から誘導されるLAK細胞の抗腫瘍効果についての実験的検討" 膵臓. 11. 231-237 (1996)

Toru Yusa：“来自胰腺癌患者的 LAK 细胞的抗肿瘤作用的实验研究”Pancreas 11. 231-237 (1996)。

小針 雅男: "膵胆道癌に対するLAK養子免疫療法の検討" 膵癌. 6. 596-604 (1992)

Masao Kobari：“胰胆管癌 LAK 过继免疫疗法的研究”《胰腺癌》，6. 596-604 (1992)。

網倉 克己: "膵癌の肝転移形成初期における基底蛋白laminin,fibronection,typeIV Collagenの関与" 膵臓. 11. 205-214 (1996)

Katsumi Amikura：“基础蛋白层粘连蛋白、纤维连接和 IV 型胶原在胰腺癌肝转移形成的早期阶段的参与”《胰腺》11. 205-214 (1996)。