Involvement of ets oncogene family in chromosomal translocation and hemopoietic cell dofferentiation into granulocyte/macrophage lineage

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1. Hemopoietic cell differentiation and ets oncogenes Relationship between ets oncogene family and hemopoietic cell differentiation was investigated. In Northern blottong analysis, expression of PU.1, one of the ets oncogene family, was enhanced by the induction of differentiation to macrophage lineage. Other members of ets family. however, did not show remarkable change. Using leukemic cells from several leukemia patients, the expression of PU.1 was detected by PCR.The expression was remarkable in leukemic cells with monocytic differentiation. These observations suggested that te detection of PU.1 message may be useful in clinical typing of leukemia.2. Cell function and ets oncogenes ets oncogenes are transcription factors, and their recognition sequence are seen in various lymphokine promoters. Mutation of ets recognition sequence abolished the transcriptional activity of those promoters. In addition, ets activated transcription cooperatively and synergistically with other factors (NF-kB of GATA). These results indicated that ets family play an important role in cell functions.3. Translocated transcription factor gene and tumorigenesis. We are now investigating the role of some transcription factors which are rearranged during leukemogenesis.