Genetic diversity of evergreen oak forest components and analysis of regeneration process based on kin analysis

基于亲缘分析的常绿栎林成分遗传多样性及更新过程分析

• 批准号: 04640612
• 负责人: KANZAKI Mamoru
• 金额: $ 1.34万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640612/
• 关键词: genetic diversity; evergreen oak forest; regeneration; isozyme; Zanthoxylum ailanthoides; polymorphism; genetic distance; gene frequency; 集団解析; 照葉樹林; 更新過程; 同位体酵素分析; 家系分析; ツクバネガシ

The genetic variation and breeding system were studied for a typical pioneer species, Zanthoxylum ailanthoides, of a evergreen oak forests. Isozyme analysis was conducted for leaf samples collected from 90 individuals growing in 13 ha permanent plot located at a evergreen oak forest in Kasugayama Forest Reserve. Clear band pattern was obtained for 16 gene Ioci, and four loci were polymorphic. There are no significant differences between observed and estimated genotype frequencies for three of polymorphic loci. The fact indicated that the inbreeding is rare in the population.The individuals were distributed contagiously and 7 distribution patches were recognized in the plot. Genetic distances among 7 groups were calculated and the cluster analysis (the UPGMA method) was applied. Adjacent groups were genetically close, and the populaton was divided into threee sub-populations being homogeneous genetically.The population was comprised of two discontinuous generations, older (about 80 yeares old) and younger (30 years old). Genetic distance between the younger generation and elder generation in same sub-populations were smaller than the other combinations.From these facts, we concluded that gene flow was spatially limited in the population even they were distributed in small area. As each population was bordered by ridges, the behavior of pollinators (bees and flower flies) and seed disperser (birds) is also affected by topography.

对万年青栎林典型先锋树种臭椿花椒的遗传变异和繁育系统进行了研究。对春日山森林保护区万年青橡树林13公顷固定样地中90个个体的叶片样品进行同工酶分析。在16个基因中有4个位点表现出多态性。三个多态位点的基因型频率观测值与估计值之间均无显著差异。结果表明，该种群内近交现象较少，种群内个体呈聚集分布，在样地内可识别出7个分布斑块。计算了7个群体间的遗传距离，并应用UPGMA法进行聚类分析。相邻群体间遗传关系密切，群体又分为3个遗传同质的亚群体，群体由老（80岁左右）和年轻（30岁左右）两个不连续世代组成。同一亚群体内的子代与子代之间的遗传距离小于其它组合，说明即使在小范围内，种群内的基因流动也是有限的。由于每个种群都以山脊为界，传粉者（蜜蜂和花蝇）和种子传播者（鸟类）的行为也受到地形的影响。