Study on the enzymatic bleaching of kraft pulp

牛皮纸浆酶法漂白的研究

• 批准号: 04660191
• 负责人: KONDO Ryuichiro
• 金额: $ 1.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660191/
• 关键词: Enzyme bleaching; Kraft pulp; Phanerochaete sordida; Brightness; Manganese peroxidase; Phanerochaete sordida; マンガンペルオキシターゼ; 非塩素系漂白; 木材腐朽菌; 白色腐朽菌; バイオブリーチング; ダイオキシン; リグニン分解酵素

To Clarify the role of the excreted extracellular enzymes during long-term incubation in a pulp biobleaching system with white-rot fungi, we have developed a cultivation system using a membrane filter which can prevent the direct contact between hyphae and kraft pulp, but allows extracellular enzymes to attack the kraft pulp. Phanerochaete sordida YK-624 brightened the pulp 21.4points to 54.0% brightness after 5-day in vitro treatment, which was significantly higher than phanerochaete chrysosporium and Coriolus versicolor did during 7-day treatment. These results indicate that cell-free, membrane-filtered components from the in vitro bleaching system are capable of delignifying unbleached kraft pulp. Obvious candidates for filterable reagents capable of delignifying and bleaching kraft pulp are peroxidase and phenoloxidase proteins. Secreted manganese peroxidase activity in the filterable components was substantial during the fungus YK-624 in vitro bleaching. A positive correlation between the level of manganese peroxidase and brightening of the pulp was observed.

为了阐明白腐真菌在纸浆生物漂白系统中长期培养期间分泌的胞外酶的作用，我们开发了一种使用膜过滤器的培养系统，该膜过滤器可以防止菌丝和硫酸盐纸浆之间的直接接触，但允许胞外酶攻击硫酸盐纸浆。花脸革菌YK-624离体处理5d后，纸浆白度提高21.4个百分点至54.0%，显著高于黄孢原毛平革菌和杂色革盖菌。这些结果表明，来自体外漂白系统的无细胞膜过滤组分能够使未漂白的硫酸盐浆脱木质素。能够脱木质素和漂白硫酸盐浆的可过滤试剂的明显候选物是过氧化物酶和酚氧化酶蛋白质。在真菌YK-624的体外漂白过程中，可过滤组分中分泌的锰过氧化物酶活性很高。锰过氧化物酶水平与纸浆白度呈正相关。

项目成果

近藤,隆一郎: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Engymes Excreted from white Ret Fungi in a Cultivation System Using a Membrane Filter" Applied and Environmental Microbiology. 60. (1994)

Ryuichiro Kondo：“在使用膜过滤器的培养系统中，通过从白色 Ret 真菌中排出的细胞外酶对硬木牛皮纸浆进行体外漂白”，应用和环境微生物学 60。（1994 年）。

近藤隆一郎: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a Membrane Filter." Applied and Environmental Microbiology. 60. (1994)

Ryuichiro Kondo：“使用膜过滤器在培养系统中通过白腐真菌分泌的细胞外酶对硬木牛皮纸浆进行体外漂白。”（1994 年）。

Ryuichiro KONDO: "In Vitro Bleaching of Hardwood Kraft Pulp by Extracellular Enzymes Excreted from White Rot Fungi in a Cultivation System Using a membrane Filter." Applied and Environmental Microbiology. volume 60, issue 3. (1994)

Ryuichiro KONDO：“在使用膜过滤器的培养系统中，通过白腐真菌分泌的细胞外酶对硬木牛皮纸浆进行体外漂白。”