Linkage analysis for biochemical marker loci in quail

鹌鹑生化标记位点连锁分析

• 批准号: 04660298
• 负责人: SHIBATA Takeshi
• 金额: $ 0.51万
• 依托单位: Kyushu Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660298/
• 关键词: Genetic linkage; Albumin-GC linkage; Quail; GC polymorphism; PI polymorphism; アルブミン-GCリンケージ; リンケージ(連鎖); 血清アルブミン; Gc蛋白; 電気泳動法; 多型; 遺伝子; 組換え価; アイソザイム; タンパク質多型

Serun albumin (ALB), vitamin D binding protein (GC), pre-albumin (Pre-ALB), protease inhibitor (PI), glucosephosphate isomerase (GPI), phosphogluconate dehydrogenase (PGD) and esterase-D (Es-D) polymorphisms in Japanese quail were investigated for genetic linkage analysis. ALB,Pre-ALB,GPI,PGD and Es-D polymorphisms were already demonstrared in previous articles. In this study, further GC polymorphism were observed by isoelectric focusing (IEF) and immunoblotting using rabbit antichicken GC.Three phenotypes were observed. These were controlled by two autosomal codominant alleles named Gc^A and Gc^B.Double backcrosses among seven loci were examined for the linkage analysis. The segregation data established close linkage between the Alb and Gc loci. The recombination frequency was estimated 0.014 <plus-minus> 0.006 (P<0.001). No clear signs of linkage were observed among Alb-Gc complex, Pre-alb, Pi, Gpi, Pgd and Es-D loc

对日本鹌鹑血清白蛋白（ALB）、维生素D结合蛋白（GC）、前白蛋白（Pre-ALB）、蛋白酶抑制剂（PI）、葡萄糖磷酸异构酶（GPI）、磷酸葡萄糖酸脱氢酶（PGD）和酯酶D（Es-D）基因多态性进行了遗传连锁分析。ALB、Pre-ALB、GPI、PGD和Es-D多态性已在以前的文章中证实。本研究采用兔抗鸡GC抗体，通过等电聚焦和免疫印迹进一步观察了鸡GC的多态性，共观察到三种表型。这些由两个常染色体共显性等位基因Gc^A和Gc^B控制。对七个基因座之间的双回交进行了连锁分析。分离数据建立了密切的连锁之间的Alb和Gc基因座。重组率为0.014 <plus-minus>± 0.006（P&lt;0.001）。在Alb-Gc复合物、Pre-alb、Pi、Gpi、Pgd和Es-D loc之间未观察到明显的连锁迹象

项目成果

芝田 猛・阿部 恒夫: "アガロースゲル電気泳動法によるウズラ赤血球酵素 (PG1,6PGD,PGM,Es-D)の同時検出法" Journal of Animal Genetics. 21. 33-37 (1993)

Takeshi Shibata 和 Tsuneo Abe：“琼脂糖凝胶电泳同时检测鹌鹑红细胞酶（PG1、6PGD、PGM、Es-D）的方法”《动物遗传学杂志》21. 33-37（1993）。

芝田猛・阿部恒夫: "アガロースゲル電気泳動法によるウズラ赤血球酵素(PGI,6PGD,PGM,Es-D)の同時検出法" Journal of Animal Genetics. 21. 33-37 (1993)

Takeshi Shibata 和 Tsuneo Abe：“通过琼脂糖凝胶电泳同时检测鹌鹑红细胞酶（PGI、6PGD、PGM、Es-D）的方法”《动物遗传学杂志》21. 33-37（1993）。

Shibata, T.: "A method of simultaneous detection of several erythrocyte isozymes (PGI,6PGD,PGM,Es-D) in quail by agarose gel electrophoresis" Journal of Animal Genetics. 21. 33-37 (1993)

Shibata, T.：“通过琼脂糖凝胶电泳同时检测鹌鹑中几种红细胞同工酶（PGI、6PGD、PGM、Es-D）的方法”动物遗传学杂志。