Linkage analysis for biochemical marker loci in quail

对日本鹌鹑血清白蛋白（ALB）、维生素D结合蛋白（GC）、前白蛋白（Pre-ALB）、蛋白酶抑制剂（PI）、葡萄糖磷酸异构酶（GPI）、磷酸葡萄糖酸脱氢酶（PGD）和酯酶D（Es-D）基因多态性进行了遗传连锁分析。ALB、Pre-ALB、GPI、PGD和Es-D多态性已在以前的文章中证实。本研究采用兔抗鸡GC抗体，通过等电聚焦和免疫印迹进一步观察了鸡GC的多态性。这些位点由两个常染色体共显性等位基因Gc^A和Gc^B控制。分离数据建立了密切的连锁之间的Alb和Gc基因座。重组率为0.014 <plus-minus>± 0.006（P&lt;0.001）。在Alb-Gc复合物、Pre-alb、Pi、Gpi、Pgd和Es-D loc之间未观察到明显的连锁迹象

Serun albumin (ALB), vitamin D binding protein (GC), pre-albumin (Pre-ALB), protease inhibitor (PI), glucosephosphate isomerase (GPI), phosphogluconate dehydrogenase (PGD) and esterase-D (Es-D) polymorphisms in Japanese quail were investigated for genetic linkage analysis. ALB,Pre-ALB,GPI,PGD and Es-D polymorphisms were already demonstrared in previous articles. In this study, further GC polymorphism were observed by isoelectric focusing (IEF) and immunoblotting using rabbit antichicken GC.Three phenotypes were observed. These were controlled by two autosomal codominant alleles named Gc^A and Gc^B.Double backcrosses among seven loci were examined for the linkage analysis. The segregation data established close linkage between the Alb and Gc loci. The recombination frequency was estimated 0.014 <plus-minus> 0.006 (P<0.001). No clear signs of linkage were observed among Alb-Gc complex, Pre-alb, Pi, Gpi, Pgd and Es-D loc