Measurement of heat production in skeletal muscle with HgCdTe infrared detector.

通过使用热电堆测量收缩过程中的产热，以获得有关肌肉收缩中化学机械转换机制的信息。在热电堆实验中，有一些假设，即肌肉的所有局部区域以相同的方式进行机械和热响应。可以通过热电堆获得关于在肌肉收缩期间沿着肌肉的长轴沿着产生的热的信息，但是不可能在肌肉的局部区域中这样做。目前尚不清楚（1）破伤风肌肉局部产热的时间过程是否与整个区域相同。(2)如何在肌肉表面上的热分布在休息的肌肉显示，以及它如何变化后，立即等长破伤风。(3)等长强直时产生的局部热与同一部位的力学反应有何关系，我们用碲镉汞红外探测器研制了热测量装置，并用热像仪对上述问题进行了研究。将青蛙缝匠肌（Rana catesbeiana）以原位长度安装到具有几个金属细标记的保持器上，并等距地强直2-5秒。用三个热率分量的热电堆测量肌肉中央局部区域的产热时程与整个肌肉区域的产热时程相同。在静止和强直肌肉的热分布是不均匀的沿着整个长度的肌肉。一般情况下，骨盆端和中部的局部区域有通过拉伸胫骨端的局部区域而缩短的趋势，且骨盆端和中部的等长强直产热量大于胫骨端。这些结果表明，在等长强直过程中，局部热和机械响应沿肌肉的整个长度是不均匀的沿着。

Measurements of heat production during contraction have been performed by use of a thermopile to obtain information about the mechanism of chemomechanical conversion in muscle contraction. In the thermopile experiment, there is some assumption that all the local region of muscle respond mechanically and thermally in the same manner. It is possible to obtain information about heat produced along the long axis of muscle during muscle contracion by the thermopile, but not possible to do that in the local region of muscle. It is unknown (1) whether time course of heat production in the local region of tetanized muscle is same as that in the whole region. (2) how thermal distribution on the muscle surface in the resting muscle is shown, and how it changes immediately after isometric tetanus. (3) how local heat produced during isometric tetanus relates to mechanical response of the same region.We constracted heat measurement apparatus by use of HgCdTe infrared detector and used a thermography to investigate above questions. Frog sartorius muscle (Rana catesbeiana) was mounted to the holder at in situ length with several metal fine markers and isometrically tetanized for 2-5 sec. Time course of heat production in central local region of muscle was same as that in whole region of muscle measured by the thermopile with 3 heat rate components. Thermal distributions in the resting and tetanized muscle were non-uniform along the entire length of muscle. In general, the local regions near pelvic end and central part tended to shorten by stretching those near the tibial end, and the amount of heat production during isometric tetanus was larger in the former thanin the latter. These result indicate that the local thermal and mechanical responses during isometric tetanus are not uniform along the entire length of the muscle.