Molecular mechanism of neural plastic changes in signal transduction, with special reference to the role of cGMP

神经可塑性信号转导变化的分子机制，特别参考cGMP的作用

• 批准号: 04670054
• 负责人: KOBAYASHI Haruo
• 金额: $ 1.41万
• 依托单位: Tokyo Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670054/
• 关键词: Sympathetic ganglia; Synaptic trans-mission; Protein phosphorylation; G-Kinase; C-Kinase; MARCKS; Cultured neurons; Exocytosis; 伝達物質; ムスカリン性作用; 伝達物質放出; ムスカリン性受容体

In the present study carried out in mammalian sympathetic ganglia, it was found that 1) cyclic (c) GMP was increased in response to muscarinic stimulation, 2) cGMP induced a long-lasting depolarization, 3) G-kinase activity was detected, and 4) specific G-kinase substrate of m. w. 87K was discovered. 87K protein was identified as MARCKS which was found in the brain as a specific C-kinase sub-strate. Roles of the phosphorylation of MARCKS by G- and C-kinases were discussed in terms of slow plastic changes in ganglionic synaptic transmission.Analyzes of endogenous functional proteins which were thought to be related to the exocytotic release of neurotransmitters were performed using de novo synapses formed between sympathetic neurons in primary culture.It was suggested that phosphorylation of non-muscle myosin II in the presynaptic terminals by myosin light chain kinase (MLCK) and subsequent interaction of myosin with actin might be involved in the process of neurotransmitter release.

在哺乳动物交感神经节中进行的本研究中，发现1）环（c）GMP响应于毒蕈碱刺激而增加，2）cGMP诱导持久的去极化，3）检测到G-激酶活性，和4）m. W. 87 K被发现了87 K蛋白被鉴定为MARCKS蛋白，它是脑内发现的一种特异性C激酶底物。本文讨论了G激酶和C激酶磷酸化MARCKS在神经节突触传递中的缓慢可塑性变化中的作用。利用原代培养的交感神经元之间形成的新突触进行了被认为与神经递质的胞吐释放有关的内源性功能蛋白的分析。结果表明，非磷酸化MARCKS的磷酸化可能是神经节突触传递中的一个重要因素。肌球蛋白轻链激酶（MLCK）介导的突触前终末肌球蛋白II的释放以及随后肌球蛋白与肌动蛋白的相互作用可能参与了神经递质的释放过程。

项目成果

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MOCHIDA,S.：“肌球蛋白 II 参与培养物中大鼠交感神经元之间 syuaqse fovmcd 的递质释放。”

MOCHIDA,S.: "Analysis of the mechanism for Ach release at the synapse formed between rat sympathetic neurons in culture." Microscopy Res.& Tech.29. 94-102 (1994)

MOCHIDA,S.：“培养大鼠交感神经元之间形成的突触释放乙酰胆碱的机制分析。”

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