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Intracelluar signal transduction of relaxin : comparative study with beta-adrenergic action

松弛素的细胞内信号转导：与β-肾上腺素能作用的比较研究

基本信息

批准号：
04670049
负责人：
OSA Takuro
金额：
$ 1.28万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670049/
关键词：
Mg Mn relaxin isoprenaline db cAMP 8-Br cGMP myometrium mag-fura2 forskolin protein kinase A myometrium

项目摘要

It was aimed how the relaxin action resembles and differs from cAMP-and cGMP-mediated effects. The longitudinal myometrium was taken from ovariectomized rat 72h after the injection of estradiol-benzoate. Phasic contractions were evoked in Mg-free Krebs solution by electrical stimulation. The [Mg]i was increased from 380muM in the control solution to 420muM, when the tissue was exposed to Ca-free 40mX K solution containing 15mM Mg. It was assumed that muscle cells were loaded with Mn, when exposed to 0.6mM Mn. Contractile responses to relaxin, isoprenaline, forskolin and cyclic nucleotide derivatives were compared before and after the intracellular load with Mg or Mn. Results are summarized in the table shown below.Influence of the elevation of intracellular Mg(b) of Mu(c) on the contractile inhibition caused by db cAMP and 8-bromo cGMP in comparison with that by isoprenaline, forskolin and relaxin. Upward and downward arrows indicate augmentation and attenuation of the inhibitory effects, respectively.The activation of protein kinase A is thought to be a common pathway of relaxin and adrenergic beta-action (Hsu and Sanborn, 1986). The questions are raised, how and why relaxin and db cAMP behabed differently from isoprenaline and forskolin under the influence of intracellular load with Mn.

其目的是松弛素作用与 cAMP 和 cGMP 介导的作用有何相似和不同。注射苯甲酸雌二醇72小时后，取卵巢切除大鼠的纵向子宫肌层。通过电刺激在不含镁的克雷布斯溶液中引起阶段性收缩。当组织暴露于含有 15mM Mg 的无 Ca 40mX K 溶液时，[Mg]i 从对照溶液中的 380μM 增加到 420μM。假设当暴露于 0.6mM Mn 时，肌肉细胞会负载 Mn。比较细胞内负载 Mg 或 Mn 之前和之后对松弛素、异丙肾上腺素、毛喉素和环核苷酸衍生物的收缩反应。结果总结于下表中。与异丙肾上腺素、毛喉素和松弛素相比，细胞内Mg(b)或Mu(c)的升高对db cAMP和8-溴cGMP引起的收缩抑制的影响。向上和向下箭头分别表示抑制作用的增强和减弱。蛋白激酶A的激活被认为是松弛素和肾上腺素能β作用的共同途径（Hsu和Sanborn，1986）。提出的问题是，在细胞内锰负荷的影响下，松弛素和 db cAMP 的行为如何以及为何与异丙肾上腺素和毛喉素不同。