Serum Propeptide and Intact Molecular Osteocalcin in Normal Children and Children with Growth Hormone(GH) Deficiency

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To establish a sensitive marker for bone formation we have developed a sandwich enzyme-linked immunosorbent assay for intact osteocalcin(OC) and its propeptide. Serum levels of these peptides were studied in 185 normal children, aged 4-15 yr, and in 23 GH-deficient children treated with GH.The serum levels of the propeptide in normal prepubescent children were 1.43 (〕SY.+-.〔) 0.23 (mean (〕SY.+-.〔) SE)mug/L in boys and 1.53 (〕SY.+-.〔) 0.23mug/L in girls. The peak value occurred at the age of 13 yr in boys (2.9 (〕SY.+-.〔) 0.42mug/L) and 11 yr in girls (2.34 (〕SY.+-.〔) 0.34mug/L). The serum intact OC levels in prepubescent boys and girls were 18.8 (〕SY.+-.〔) 2.1 and 20.7 (〕SY.+-.〔) 2.1mug/L, respectively, and these levels increased to 41.0 (〕SY.+-.〔) 3.7mug/L in boys aged 13 yr and to 27.0 (〕SY.+-.〔) 2.5mug/L in girls aged 11 yr. In the GH-deficient patients, a 2.3-fold increase in the propeptide levels and a 1.7-fold increase in the intact OC level was observed after 1 month of GH therapy. Serum propeptide and intact OC levels after 1 month of GH therapy correlated with the growth response after 12 months of GH therapy(r=0.660 and P<0.01, for propeptide ; r=0.537 and P<0.01 for intact OC).