An assessment of tissue perfusion of intestinal with non-contact type colorimeter

非接触式比色计评估肠道组织灌注

• 批准号: 04670736
• 负责人: OHNISHI Katsuyuki
• 金额: $ 1.28万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670736/
• 关键词: colic ischemia; assessment of intestinal tissue perfusion; colorimeter; 色彩色差計; 結腸虚血; 家兎; 腹部大動脈血行再建

PURPOSE : Various methods for estimating colic ischemia have been reported. At present, however surgeon use the color tone on the serous surface of the colon in making a final judgment. In this experiment, we made various models of clamped blood vesseles feeding the rabbit sigmoid colon and expressed the shade of the serious surface numerically with a non-contact colorimeter to objectively evaluate tissue perfusion of the intestinal.METHODS : Using rabbits weighing 2 to 3 Kg (n=10), various arteries : (1) caudal mesenteric artery, (2) bilateral internal iliac artery and (3) cranial mesenteric artery were clamped and the animals were divided into three groups, namely Group1 : only (1) clamped, Group2 : (1)+(2) clamped and Group3 : (1)+(2)+(3) clamped. The hue (a ^<**> : in the direction of red-green, b^<**> : in the direction of yellow-blue) and chroma(C^<**>) were measured woth a colorimeter (Minolta Co.-make CS-100) before (control group) and 5 minutes after clamping, and the values m … More easured were processed with a data processor (DP-101). Then, a comparative study was between each clamped groups and the control group. Additionally, the tissue blood flow in the same region was measured with a laser doppler flowmeter(T.S.I.Co-make BPM 403).RESULTS : a ^<**> : Control 36.1(〕SY.+-.〔) 3.3, Group 1 28.2(〕SY.+-.〔) 4.2, Group 2 27.9(〕SY.+-.〔) 4.7, Group 3 22.7(〕SY.+-.〔) 4.3, b^<**> : Control 18.2 (〕SY.+-.〔) 3.3, Group 1 13.6 (〕SY.+-.〔) 2.9, Group 2 12.7 (〕SY.+-.〔) 2.9, Group 3 9.8 (〕SY.+-.〔) 2.3, C^<**> : Control 40.5 (〕SY.+-.〔) 3.6, Group 1 31.8 (〕SY.+-.〔) 5.1, Group 2 30.7 (〕SY.+-.〔) 5.3, Group 3 24.8 (〕SY.+-.〔) 4.5. a^<**>, b^<**> andC^<**>decreased significantly in every clamped group compared with the control group(p<0.05). There was a positive significant correlation between the chroma and tissue blood flow, the coefficient being 0.79(p<0.01).CONCLUSION : 1. The hue and chroma decreased significantly with the clamping of blood flow in any clamped groups compared with the control group. 2. The chroma was significant correlated with changes in the tissue blood flow. 3. We succeeded in numerically expressing the shades to objectively evaluate the tissue perfusion of the colon after clamped various blood flow in rabbits by using a no-contact colorimeter. This approach is considered usuful as a new method for assessment of intestinal tissue perfusion. Less

目的：已经报道了各种评估结肠缺血的方法。然而，目前，外科医生使用结肠浆液表面的色调来做出最终判断。本实验采用兔乙状结肠血管夹闭模型，用非接触式比色计对乙状结肠血管表面的色度进行数值表示，以客观评价乙状结肠的组织灌注情况。（1）夹闭肠系膜尾动脉、（2）夹闭双侧髂内动脉和（3）夹闭肠系膜前动脉，并将动物分为3组，即组1：仅夹闭（1），组2：第3组：（1）+（2）+（3）夹闭。用色度计（Minolta Co.，Ltd.）测量色调（a ^**>：在红-绿方向上，B^**>：在黄-蓝方向上）和色度（C^**>）。CS-100），并分别于夹闭前（对照组）和夹闭后5 min测定其m ...更多信息 用DP-101数据处理机处理。然后将各夹闭组与对照组进行比较研究。另外，用激光多普勒流量计（T. S. I. Co-make BPM 403）测量相同区域中的组织血流。（）3.3，组1 28.2（）SY. ±.（）4.2，组2 27.9（）SY. ±.（）4.7，第3组22.7（）SY ±。（）4.3，B^<**>：对照18.2（）SY. ±.（）3.3，组1 13.6（）SY. ±.（）2.9，第2组12.7（）SY ±。（）2.9，第3组9.8（）SY. ±.（）2.3，C（**）：对照40.5（）SY. ±.（）3.6，组1 31.8（）SY. ±.（）5.1，组2 30.7（）SY. ±.（）5.3，第3组24.8（）SY ±。（）4.5.与对照组相比，各钳夹组a^<**>、B^<**>、C ^<**>均显著降低（p<0.05）。色度与组织血流量呈显著正相关，相关系数为0.79（p<0.01）。与对照组相比，各夹闭组的色调和色度均随着血流的夹闭而明显下降。2.色度与组织血流量的变化显著相关。3.我们成功地用数值表示色度，以客观地评价兔结肠在各种血流阻断后的组织灌注。这种方法被认为是一种评价肠组织灌注的新方法。少

项目成果

朝日俊明,大西克幸,小野敦美,清地秀典,小林展章: "腸管壁の色調による血行動態の評価" 血管無侵襲診断法研究会誌. 13. 37-38 (1993)

Toshiaki Asahi、Katsuyuki Onishi、Atsumi Ono、Hidenori Kiyochi、Nobuaki Kobayashi：“基于肠壁色调的血流动力学评估”无创血管诊断学会杂志 13. 37-38 (1993)。