An assessment of tissue perfusion of intestinal with non-contact type colorimeter

目的：各种评估绞痛缺血的方法已有报道。然而，目前外科医生使用结肠浆膜表面的色调来做出最终判断。本实验制作了兔乙状结肠供血血管夹闭模型，并用非接触式比色仪用数字表示严重表面的阴影，以客观评价肠道的组织灌注量。方法：选用体重2~3 kg(n=10)的兔，分别夹闭肠系膜尾动脉、双侧髂内动脉和颅肠系膜动脉，分为3组，即1组：仅(1)夹，2组：(1)+(2)夹，3组：(1)+(2)+(3)夹。在夹闭前(对照组)和夹闭后5分钟，用美能达公司的色度计(美能达公司生产的CS-100)测量色调(红-绿方向，黄蓝方向)和色度(C^&lt；**&gt；)，数值m…用数据处理器(DP-101)处理更多的数据。然后，将各夹闭组与对照组进行比较研究。结果：对照组36.1()sy.+.()3.3，组1 28.2()sy.+-4.2，组2 27.9()sy.+-4.7，组3 22.7()sy.+-4.3；**&gt；：对照18.2()sy.+-.()3.3，组1 13.6()sy.+-.()2.9，组2 12.7()sy.+-.()2.9，组3 9.8()sy.+-.()2.3，C^&lt；**&gt；：对照40.5()sy.+-.()3.6，组1 31.8()sy.+-.()5.1，组2 30.7()sy.+-.()5.3，组3 24.8()sy.+-.()4.5。与对照组相比，各钳夹组的a^&lt；**&gt；、b^&lt；**&gt；和dc^&lt；**&gt；均显著降低(p&lt；0.05)。色度与组织血流量呈显著正相关，相关系数为0.79(p&lt；0.01)。结论：1.随着血流阻断，各夹闭组的色度和色度均较对照组明显降低。2.色度与组织血流量的变化显著相关。3.利用非接触式比色仪，成功地用数值表达的方法客观地评价了兔不同血流阻断后结肠的组织灌注量。这种方法被认为是一种评估肠道组织灌注度的新方法。较少

PURPOSE : Various methods for estimating colic ischemia have been reported. At present, however surgeon use the color tone on the serous surface of the colon in making a final judgment. In this experiment, we made various models of clamped blood vesseles feeding the rabbit sigmoid colon and expressed the shade of the serious surface numerically with a non-contact colorimeter to objectively evaluate tissue perfusion of the intestinal.METHODS : Using rabbits weighing 2 to 3 Kg (n=10), various arteries : (1) caudal mesenteric artery, (2) bilateral internal iliac artery and (3) cranial mesenteric artery were clamped and the animals were divided into three groups, namely Group1 : only (1) clamped, Group2 : (1)+(2) clamped and Group3 : (1)+(2)+(3) clamped. The hue (a ^<**> : in the direction of red-green, b^<**> : in the direction of yellow-blue) and chroma(C^<**>) were measured woth a colorimeter (Minolta Co.-make CS-100) before (control group) and 5 minutes after clamping, and the values m … More easured were processed with a data processor (DP-101). Then, a comparative study was between each clamped groups and the control group. Additionally, the tissue blood flow in the same region was measured with a laser doppler flowmeter(T.S.I.Co-make BPM 403).RESULTS : a ^<**> : Control 36.1(〕SY.+-.〔) 3.3, Group 1 28.2(〕SY.+-.〔) 4.2, Group 2 27.9(〕SY.+-.〔) 4.7, Group 3 22.7(〕SY.+-.〔) 4.3, b^<**> : Control 18.2 (〕SY.+-.〔) 3.3, Group 1 13.6 (〕SY.+-.〔) 2.9, Group 2 12.7 (〕SY.+-.〔) 2.9, Group 3 9.8 (〕SY.+-.〔) 2.3, C^<**> : Control 40.5 (〕SY.+-.〔) 3.6, Group 1 31.8 (〕SY.+-.〔) 5.1, Group 2 30.7 (〕SY.+-.〔) 5.3, Group 3 24.8 (〕SY.+-.〔) 4.5. a^<**>, b^<**> andC^<**>decreased significantly in every clamped group compared with the control group(p<0.05). There was a positive significant correlation between the chroma and tissue blood flow, the coefficient being 0.79(p<0.01).CONCLUSION : 1. The hue and chroma decreased significantly with the clamping of blood flow in any clamped groups compared with the control group. 2. The chroma was significant correlated with changes in the tissue blood flow. 3. We succeeded in numerically expressing the shades to objectively evaluate the tissue perfusion of the colon after clamped various blood flow in rabbits by using a no-contact colorimeter. This approach is considered usuful as a new method for assessment of intestinal tissue perfusion. Less