A study of the effectiveness of a carcinostatic and increasing carcinostatic tolerance

本研究的目的有三个方面：1.制备抗癌抗体；2)应用抗癌抗体以减轻抗癌药物的副作用；3)分析抗癌作用的增强作用；3.制备抗癌单抗。实验选用两组Wistar大鼠。一组仅接受ADM治疗，另一组接受ADM+抗ADM抗体治疗，分析比较两组患者的心脏和骨髓功能。ADM加抗体组的心功能障碍减轻，骨髓检查显示白细胞和血小板水平保持不变。观察到与抗癌相关的副作用显著减轻。NEX…更多的目标将是确定抗癌抗体的正确剂量和最佳给药时机。(在本实验中，抗体是在抑癌后90分钟注射的)。我们还希望制备一种单抗和其他抗癌药物的抗体。然后我们检查了一种抗癌药物的有效性和耐受性。目前还没有一种有效的媒介来增加对抗癌药物的耐受性。短时间重复使用抗癌药物是获得合理耐受性的最理想方法。实现这种反复给药的一种方法是中和药物的影响，这将使副作用得到缓解。因此，我们可以增加药物的有效性。事实上，在ADM+抗体组中观察到了比单独使用ADM组更显著的抗癌效果。最后，制备了一种单抗。作者成功地获得了一种与ADM抗原具有特异性反应的IgG型单抗，该单抗应该比以往使用的多克隆抗体具有更高的特异性，下一步的实验将专注于提高新抗体的特异性。较少

A STUDY OF THE EFFECTIVENESS OF A CARCINOSTATIC AND INCREASING CARCINOSTATIC TOLERANCESummary of the study(for the past fiscal year)The purpose of the study was threefold :1.to prepare an antibody anti-carcinostatic2.to administer the antibody anti-carcinostatica)to relieve side effects of the carcinostaticb)to analyze the potentiation of the carcinostatic effect3.to prepare a carcinostatic monoclonal antibodyOver the fiscal year, the author concentrated on analyzing the alleviation of carcinostatic related side effects. Two groups of Wistar rats were used for the experiment. One group received ADM only, while the other received ADM followed by an antibody anti-ADM, Heart and bone marrow functions in these two groups were then analyzed and compared. Heart functional disorders in the ADM plus antibody group were reduced, and the bone marrow study showed that leukocyte and thrombocyte levels remained unchanged. Significant easing of carcinostatic-related side effects was observed.The nex … More t objective will be to determine the correct dosage of the antibody anti-carcinostatic and the optimal timing for administration. (The antibody was administered 90 minutes after the carcinostatic in this experiment). We also hope to prepare a MoAb and antibodies for other carcinostatics.We then examined the effectiveness and tolerance levels of a carcinostatic. There is not yet an effective medium for increasing tolerance to carcinostatic drugs. Repeated administration of the carcinostatic at short intervals is the most desirable means of obtaining reasonable increases in tolerance. One way to achieve such repeated administration would be to neutralize the drug's effects, which would allow side effects to be relieved. Thus, we can increase the effectiveness of the drug. Indeed, a more significant anti-cancer effect was observed in the ADM plus antibody group the ADM-alone group.Finally, a monoclonal antibody was prepared. The author succeeded in obtaining an IgG-type monoclonal antibody which has a specific reaction against the antigen, ADM.This monoclonal antibody should be more specific than polyclonal antibodies used in the past, and the next experiments will focus on increasing the specificity of the new antibody. Less