Development of the Screening System for Adrenal Hypertension

A)建立原发性醛固酮增多症和糖皮质激素可修复性醛固酮增多症(1)自动化酶免疫分析筛选系统，采用多克隆抗体包被管和过氧化物酶标记的18-羟基皮质醇。实验结果如下：a)我们确认滤纸上的干尿斑可以用这种方法检测。b)我们证实了即时尿液对原发性醛固酮增多症的诊断是有用的。c)我们已经成功地筛选了糖皮质激素可补救醛固酮增多症谱系中的受影响成员。2)采用单克隆抗体包被的磁载体和吖啶酸酯标记的18-羟基皮质醇，建立了自动化学发光免疫分析法。检测结果如下：a)检测时间由3 h缩短至15 min，灵敏度提高10倍。b)该系统包括单克隆抗体已申请专利。B)嗜铬细胞瘤筛选体系的研究将香草酸与牛血清白蛋白结合物免疫小鼠，建立了高特异性、高亲和力的单克隆抗体。化学发光免疫分析法正在研制中。

A) Reseacrh of the screening system for primary aldosteronism and glucocorticoid-remediable aldosteronism1) antomated enzyme immunoassay, using polyclonal antibody-coated tube and peroxidase-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) We confirmed that dry urin spot on the filter paper can be assayd by this method.b) We confirmed that spot-time urine is useful for the diagnosis of primary aldosteronism.c) We have succeeded in screening an affected member in a pedigree of glucocorticoid-remediable aldosteronism.2) automated chemiluminescence immunoassay, using monoclonal antibody-coated magnet support and acridinum ester-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) Assay time was shortened from 3 h to 15 min, and senseitivity was improved 10-fold.b) This system including the monoclonal antibodies has been submitted for patent.B) Research of the screening system for pheochromocytomaThe conjugate of vanillylmandelic acid and bovine serum albumin was immunized in mouse, and monoclonal antibodies with high specificity and affinity have been established.Chemiluminescence immunoassay is being prepared.