Development of the Screening System for Adrenal Hypertension

肾上腺高血压筛查系统的开发

• 批准号: 04670946
• 负责人: CHIBA Hitoshi
• 金额: $ 1.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670946/
• 关键词: Primary aldosteronism; Glucocorticoid-remediable aldosteronsim; 18-hydroxycortisol; Mass-screening; Pheochromocytoma; Vanillyl mandelic acid; vanillylmanderic acid; 18‐hydroxycortisol; 化学発光; イムノアッセイ

A) Reseacrh of the screening system for primary aldosteronism and glucocorticoid-remediable aldosteronism1) antomated enzyme immunoassay, using polyclonal antibody-coated tube and peroxidase-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) We confirmed that dry urin spot on the filter paper can be assayd by this method.b) We confirmed that spot-time urine is useful for the diagnosis of primary aldosteronism.c) We have succeeded in screening an affected member in a pedigree of glucocorticoid-remediable aldosteronism.2) automated chemiluminescence immunoassay, using monoclonal antibody-coated magnet support and acridinum ester-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) Assay time was shortened from 3 h to 15 min, and senseitivity was improved 10-fold.b) This system including the monoclonal antibodies has been submitted for patent.B) Research of the screening system for pheochromocytomaThe conjugate of vanillylmandelic acid and bovine serum albumin was immunized in mouse, and monoclonal antibodies with high specificity and affinity have been established.Chemiluminescence immunoassay is being prepared.

（1）建立了原发性醛固酮增多症和糖皮质激素可治疗性醛固酮增多症的筛选系统。（2）应用多克隆抗体包被管和过氧化物酶标记的18-羟基皮质醇建立了自动酶免疫分析法。结果表明：（1）滤纸干尿斑法可用于原发性醛固酮增多症的诊断;（B）尿斑法可用于原发性醛固酮增多症的诊断;（3）成功地筛选了一个糖皮质激素可治疗的醛固酮增多症家系成员;（4）建立了以吖啶酯标记18-羟基皮质醇的单克隆抗体包被的磁性载体化学发光免疫分析法。结果表明：（1）检测时间由3 h缩短到15 min，灵敏度提高了10倍;（B）建立的单克隆抗体检测体系已申请专利;（B）嗜铬细胞瘤筛选体系的研究香草扁桃酸-牛血清白蛋白偶联物免疫小鼠，制备了特异性和亲和力高的单克隆抗体，并制备了化学发光免疫分析法。

项目成果

千葉仁志: "原発性アルドステロン症のスクリーニング法" 臨床化学. 21補2. 12b-13b (1992)

千叶仁：“原发性醛固酮增多症的筛查方法”临床化学21增刊2.12b-13b(1992)。

千葉 仁志: "原発性アルドステロン症のスクリーニング法" 臨床化学. 21補2. 12b-13b (1992)

Hitoshi Chiba：“原发性醛固酮增多症的筛查方法”临床化学 21 Suppl. 2. 12b-13b (1992)

Kohno H: "An enzyme immunoassay,using a monoclonal antibody,for urinary 18-hydroxycortisol" Clinical biochemistry. (submitted).

Kohno H：“酶免疫测定法，使用单克隆抗体，用于尿 18-羟基皮质醇”临床生物化学。

Chiba H: "A direct enzyme immunoassay for 18-hydroxycortisol" Journal of Steroid Biochemistry and molecular Biology. 46. 85-89 (1993)

Chiba H：“18-羟基皮质醇的直接酶免疫测定”类固醇生物化学和分子生物学杂志。

Chiba H: "A direct enzyme immunoassay for 18‐hydroxycortisol in urine:a new tool for screening primary aldosteronism" Journal of Steroid Biochemistry and Molecular Biology. 46. (1993)

Chiba H：“尿液中 18-羟基皮质醇的直接酶免疫测定：筛查原发性醛固酮增多症的新工具”类固醇生物化学与分子生物学杂志 46。（1993）