Development of the Screening System for Adrenal Hypertension

A) 研究原发性醛固酮增多症和糖皮质激素可治疗的醛固酮增多症筛查系统1) 建立了使用多克隆抗体包被管和过氧化物酶标记的18-羟基皮质醇的自动酶免疫测定法。以下是测定结果：a) 我们证实滤纸上的干尿斑点可通过此方法进行测定。b) 我们证实点时尿液可用于诊断原发性醛固酮增多症。c) 我们已成功筛选出糖皮质激素可治愈的醛固酮增多症家系中受影响的成员。2) 使用单克隆抗体包被的磁体支持物和吖啶酯标记的自动化学发光免疫测定法18-羟基皮质醇成立。检测结果如下：a) 检测时间由3小时缩短至15分钟，灵敏度提高10倍。b) 该单克隆抗体系统已申请专利。B) 嗜铬细胞瘤筛查系统的研究将香草扁桃酸与牛血清白蛋白的缀合物免疫小鼠，建立了具有高特异性和亲和力的单克隆抗体。正在准备中。

A) Reseacrh of the screening system for primary aldosteronism and glucocorticoid-remediable aldosteronism1) antomated enzyme immunoassay, using polyclonal antibody-coated tube and peroxidase-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) We confirmed that dry urin spot on the filter paper can be assayd by this method.b) We confirmed that spot-time urine is useful for the diagnosis of primary aldosteronism.c) We have succeeded in screening an affected member in a pedigree of glucocorticoid-remediable aldosteronism.2) automated chemiluminescence immunoassay, using monoclonal antibody-coated magnet support and acridinum ester-labeled 18-hydroxycortisol, was established. Following is the results obtained by the assay :a) Assay time was shortened from 3 h to 15 min, and senseitivity was improved 10-fold.b) This system including the monoclonal antibodies has been submitted for patent.B) Research of the screening system for pheochromocytomaThe conjugate of vanillylmandelic acid and bovine serum albumin was immunized in mouse, and monoclonal antibodies with high specificity and affinity have been established.Chemiluminescence immunoassay is being prepared.