The basic reseach in clinical field with alpha-blocker and beta-stimulant therapy for male infertility

α受体阻滞剂和β兴奋剂治疗男性不育症的临床基础研究

• 批准号: 04670959
• 负责人: MIYAKE Koji
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670959/
• 关键词: epididymis; sperm parameter; a-blocker; α-遮断剤; 精子濃度; 精巣; 微小穿刺

This study was undertaken to investigate whether alpha blocker has an effect on epididymal sperm parameters, intraluminal pressure and fluid movement in the rat cauda epididymal tubules. Sprague Dawley rats were given daily oral doses 1mg/kg, 2mg/kg, or 10mg/kg of Bunazosin, which was a selective alpha blocker, for one month. Intraluminal fluids were collected from the cauda epididymidis using a micropuncture technique. Epididymal sperm concentrations were significantly increased after administration of alpha blocker. Progressive motility of apermatozoa was not significantly altered by alpha bloker. Intraluminal pressures were significantly reduced and intraluminal fluid movement rates were significantly increased by alpha blocker. Outside diameters of seminiferous and epididymal tubules were significantly distended when treated with alpha-blocker.The increase in epididymal sperm concentration after alpha blocker administration seems to be associated with dilatation of the epididymal tubules that results in subsequente maintenance of reduced luminal pressure coupled with fast tubular fluid flow and increased sperm storage capacity. This research is a first step for additional investigations of the role of alpha-blocker in testicular or epididymal physiology and may pave way for human clinical application of alpha-blocker therapy in the treatment of male infertility.

本研究旨在探讨α受体阻滞剂是否对大鼠附睾尾小管精子参数、管腔内压力和液体运动有影响。Sprague道利大鼠每天口服1 mg/kg、2 mg/kg和10 mg/kg的选择性α受体阻滞剂布纳唑嗪，持续1个月。使用显微穿刺技术从附睾尾收集管腔内液体。α受体阻滞剂给药后，附睾精子浓度显著增加。α阻断剂没有显著改变精子的运动性。α受体阻滞剂显着降低了腔内压力，显着增加了腔内液体运动速率。用α-受体阻滞剂处理后，生精小管和附睾小管的外径显著扩张，α-受体阻滞剂给药后附睾精子浓度的增加似乎与附睾小管的扩张有关，这导致了管腔压力的降低，以及小管内液体的快速流动和精子储存能力的增加。这项研究是进一步研究α-受体阻滞剂在睾丸或附睾生理学中作用的第一步，可能为α-受体阻滞剂治疗男性不育症的人类临床应用铺平道路。

项目成果

Yamamoto M,Tsuji Y,Hibi H and Miyake.K: "Epididymal sperm concentration determined by micropuncture technique and daily testicular sperm production in the rat." Jpn J Fertil Steril. 39. 229-232 (1994)

Yamamoto M、Tsuji Y、Hibi H 和 Miyake.K：“通过微穿刺技术和大鼠每日睾丸精子产生测定附睾精子浓度。”

Yamamoto M.et.al.: "Effects of protein synthesis inhibitor and antimicrotubular agent on transepithelial movement of ^3H-androgens in the rat caput epididymis." Nagoya J.Med.Sci.(in press).

Yamamoto M.et.al.：“蛋白质合成抑制剂和抗微管剂对大鼠附睾中^3H-雄激素跨上皮运动的影响。”

YAMAMOTO,M.et al.: "Microassay of Sperm Concentration in the rat epididymis by micropumcture technique." Nagoya.J.Med.Sci.(1993)

YAMAMOTO,M.et al.：“通过微穿刺技术对大鼠附睾中的精子浓度进行微量测定。”

Yamamoto M.et.al.: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J.Med.Sci.55. 71-75 (1993)

Yamamoto M.et.al.：“通过微穿刺技术对大鼠附睾中的精子浓度进行微量测定。”

Yamamoto M,Tsuji Y and Miyake K: "Microassay of sperm concentration in the rat epididymis by micropuncture technique." Nagoya J Med Sci. 55. 71-75 (1993)

Yamamoto M、Tsuji Y 和 Miyake K：“通过微穿刺技术对大鼠附睾中的精子浓度进行微量测定”。