The basic reseach in clinical field with alpha-blocker and beta-stimulant therapy for male infertility

本研究旨在探讨α受体阻滞剂是否对大鼠附睾尾小管精子参数、管腔内压力和液体运动有影响。Sprague道利大鼠每天口服1 mg/kg、2 mg/kg和10 mg/kg的选择性α受体阻滞剂布纳唑嗪，持续1个月。使用显微穿刺技术从附睾尾收集管腔内液体。α受体阻滞剂给药后，附睾精子浓度显著增加。α阻断剂没有显著改变精子的运动性。α受体阻滞剂显著降低了腔内压力，显著增加了腔内液体运动速率。用α-受体阻滞剂处理后，生精小管和附睾小管的外径显著扩张，α-受体阻滞剂给药后附睾精子浓度的增加似乎与附睾小管的扩张有关，这导致了管腔压力的降低，以及小管内液体的快速流动和精子储存能力的增加。这项研究是进一步研究α-受体阻滞剂在睾丸或附睾生理学中作用的第一步，可能为α-受体阻滞剂治疗男性不育症的人类临床应用铺平道路。

This study was undertaken to investigate whether alpha blocker has an effect on epididymal sperm parameters, intraluminal pressure and fluid movement in the rat cauda epididymal tubules. Sprague Dawley rats were given daily oral doses 1mg/kg, 2mg/kg, or 10mg/kg of Bunazosin, which was a selective alpha blocker, for one month. Intraluminal fluids were collected from the cauda epididymidis using a micropuncture technique. Epididymal sperm concentrations were significantly increased after administration of alpha blocker. Progressive motility of apermatozoa was not significantly altered by alpha bloker. Intraluminal pressures were significantly reduced and intraluminal fluid movement rates were significantly increased by alpha blocker. Outside diameters of seminiferous and epididymal tubules were significantly distended when treated with alpha-blocker.The increase in epididymal sperm concentration after alpha blocker administration seems to be associated with dilatation of the epididymal tubules that results in subsequente maintenance of reduced luminal pressure coupled with fast tubular fluid flow and increased sperm storage capacity. This research is a first step for additional investigations of the role of alpha-blocker in testicular or epididymal physiology and may pave way for human clinical application of alpha-blocker therapy in the treatment of male infertility.