The cellular immuno-chemistrical study of intraperitoneal environment in patients with endometriosis.

最近有研究表明，前列腺素（PG）的生物合成在子宫内膜异位症妇女的腹膜液中增加。为评价子宫内膜异位症患者腹膜液细胞（PFC）的PG生成，测定了PFC细胞的磷脂酶A_2活性，并与正常子宫内膜异位症患者进行了比较。细胞中至少检测到4种可测的磷脂酶A_2活性，包括2种钙依赖性pH值7.0和9.0的活性，以及2种钙非依赖性pH值7.5和8.5的活性。其中一种活性，即钙依赖性碱性（pH 9.0）活性酶，在子宫内膜异位症女性（n=15）中，无论在蛋白质基础上还是在细胞数量上，都明显高于无子宫内膜异位症女性（n=9, p<0.01）。提示子宫内膜异位症腹膜液中前列腺素水平升高可能是由于PFC导致磷脂酶A_2活性升高所致。

It has recently been indicated that prostaglandin (PG) biosynthesis increases in the peritoneal fluid of women with endometriosis. In order to assess the PG production on peritoneal fluid cells (PFC), phospholipase A_2 activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis. There were at least four measurable kinds of phospholipase A_2 activity detected in the cells, such as two calcium-dependent pH optima 7.0 and 9.0 activities as well as two calcium-independent pH optima 7.5 and 8.5 activities. One of those activities, the calcium-dependent and alkaline(pH 9.0) active enzyme, was significantly higher in women with endometriosis (n=15) as cmpared with that in women without endometriosis(n=9, p<0.01) both on a protein basis and cell number basis. These results indicate that the elevated prostaglandin levels in peritoneal fluid with endometriosis may be produced by PFC in which the phospholipase A_2 activity is elevated.