Purification of an enzyme separating outer layr of the cell wall of Micrococcus luteus

从野生型菌株IFO 3333的培养上清液中分离到一种具有抑制黄微球菌突变株MT胞外蛋白质形成的活性物质。经Q-SepharoseFF和Sephacryl S-200HR超滤和柱层析，得到的蛋白质在天然聚丙烯酰胺凝胶电泳(PAGE)上是均一的。在SDS-PAGE中，该蛋白出现一条相对分子质量约为200,000的单一蛋白条带和多条相对分子质量小于30,000的条带。扫描电子显微镜显示，许多颗粒均匀地附着在MT细胞的四分体表面，经该蛋白培养后，四分体表面的包形成受到抑制。

An extracellular protein which showed activity inhibiting packets formation of Micrococcus luteus mutant strain MT was isolated from the culture supernatant of wild type strain IFO 3333.The protein obtained by ultrafiltration and column chromatographies with Q-Sepharose FF and Sephacryl S-200 HR was homogeneous on native polyacrylamide gel electrophoresis (PAGE). In SDS-PAGE, the protein gave a single protein band corresponding to a molecular weight of about 200,000 and several bands corresponding to molecular weights of less than about 30,000.Scanning electron microscopy revealed many particles attaching uniformly to the tetrads surface of MT cells whose packets formation was inhibited by cultivation with the protein.