Effects of Volatile Anesthetics on the Intracellular Ca^<2+> Concentration

挥发性麻醉药对细胞内Ca^<2>浓度的影响

• 批准号: 04671227
• 负责人: KAWAHARA Michio
• 金额: $ 1.15万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671227/
• 关键词: Guinea pig; Isolated hepatocytes; Intracellular Ca^<2+> concentration; Halothane; Isoflurane; Sevoflurance; 細胞内CA^<2+>濃度

The effects of halothane on the intracellular Ca^<2+> concentration ([Ca^<2+>]i) of isolated guinea pig hepatocytes were investigated .When hepatocytes were incubated in Hank's solution containing Ca^<2+>, trypan blue uptake which indicates death of cell was 13.5<plus-minus>3.41%, and [Ca^<2+>]i was 158<plus-minus>73.5nM in control.By halothane exposure, both trypan blue uptake and [Ca^<2+>]i were increased in concentration-dependent fashion.When hepatocytes were incubated in Hank's solution excluded Ca^<2+>, trypan blue uptake was 31.2<plus-minus>6.40%, and [Ca^<2+>]i was 249<plus-minus>27.5nM in control.By halothane exposure, trypan blue uptake was increased in concentration-depandent fashion, while [Ca^<2+>]i was decreased.From these results, the increases of trypan blue uptake in experiments 1 and 2 indicate the hepatotoxicity of halothane with or without Ca^<2+> in medium. The increase of [Ca^<2+>]i of hepatocytes in physiological condition indicate the direct injury of halothane to hepatocyte cell membrane.

研究了氟烷对豚鼠离体肝细胞胞内Ca^2+浓度([Ca^2+]i)的影响。肝细胞在含Ca^2+的Hank's溶液中孵育时，表明细胞死亡的台盼蓝摄取率为13.5±3.41%，[Ca^2+]i为 对照为158±73.5nM。通过氟烷暴露，台盼蓝摄取和[Ca^2+]i均以浓度依赖性方式增加。当肝细胞在排除Ca^2+的Hank's溶液中培养时，台盼蓝摄取为31.2±6.40%，[Ca^2+]i为 对照中为249±27.5nM。通过氟烷暴露，台盼蓝摄取以浓度依赖性方式增加，而[Ca^<2+>]i减少。从这些结果来看，实验1和2中台盼蓝摄取的增加表明培养基中含有或不含Ca^2+的氟烷的肝毒性。生理状态下肝细胞[Ca^2+]i的增加表明氟烷对肝细胞细胞膜的直接损伤。

项目成果

H.Kinugawa, M.Kawahara: "Halothane-Induced Cytotoxicity in Isolated Rat Hepatocytes" IN VIVO. 7. 497-502 (1993)

H.Kinukawa、M.Kawahara：“氟烷诱导的离体大鼠肝细胞的细胞毒性”体内。

H.Kinugawa, M.Kawahara: "Effects of Halothane on the Intracellular Ca^<2+> Concentration of Isolated Guinea Pig Hepatocytes" Hiroshima Journal of Anesthesia. 29. 17-19 (1993)

H.Kinukawa、M.Kawahara：“氟烷对离体豚鼠肝细胞内 Ca^<2> 浓度的影响”广岛麻醉杂志。

絹川裕史: "モルモット分離肝細胞内Ca^<2+>動態におよぼすハロセンの影響" 日本歯科麻酔学会雑誌. 23-2(掲載予定). (1995)

Hiroshi Kinukawa：“氟烷对豚鼠离体肝细胞 Ca^2+ 动态的影响”，日本牙科麻醉学会杂志 23-2（待出版）。

絹川裕史: "肝細胞内Ca^<2+>動態におよぼすハロセンの影響" 麻酔と蘇生 第29巻別冊第30号麻酔薬代謝と臓器障害. XIV. 17-19 (1993)

Hiroshi Kinukawa：“氟烷对细胞内 Ca^<2+> 动力学的影响”麻醉和复苏第 29 卷特刊第 30 号麻醉代谢和器官疾病 XIV。

絹川裕史: "肝細胞内Ca^<2+>動態におよぼすハロセンの影響" 麻酔と蘇生第29巻別刷、麻酔薬代謝と臓器障害. XIV. 17-19 (1993)

Hiroshi Kinukawa：“氟烷对细胞内 Ca^2+ 动力学的影响”麻醉和复苏第 29 卷重印，麻醉代谢和器官疾病 XIV。