Effects of Volatile Anesthetics on the Intracellular Ca^<2+> Concentration

本文研究了氟烷对豚鼠肝细胞内游离钙离子浓度的影响。在含钙的Hank‘s液中培养的豚鼠肝细胞，台盼蓝摄取率为13.5+lt；正负3.41%，对照组为158&lt；正负-gt；73.5 nM。在不含钙的Hank‘s液中孵育肝细胞，台盼蓝摄取率为31.2+-6.40%，对照组为249；+-27.5 nM。结果表明，实验1和实验2的台盼蓝摄取增加提示氟烷对肝细胞的毒性作用。生理状态下肝细胞内[Ca~(2+)]~(2+)浓度升高，提示氟烷对肝细胞膜有直接损伤作用。

The effects of halothane on the intracellular Ca^<2+> concentration ([Ca^<2+>]i) of isolated guinea pig hepatocytes were investigated .When hepatocytes were incubated in Hank's solution containing Ca^<2+>, trypan blue uptake which indicates death of cell was 13.5<plus-minus>3.41%, and [Ca^<2+>]i was 158<plus-minus>73.5nM in control.By halothane exposure, both trypan blue uptake and [Ca^<2+>]i were increased in concentration-dependent fashion.When hepatocytes were incubated in Hank's solution excluded Ca^<2+>, trypan blue uptake was 31.2<plus-minus>6.40%, and [Ca^<2+>]i was 249<plus-minus>27.5nM in control.By halothane exposure, trypan blue uptake was increased in concentration-depandent fashion, while [Ca^<2+>]i was decreased.From these results, the increases of trypan blue uptake in experiments 1 and 2 indicate the hepatotoxicity of halothane with or without Ca^<2+> in medium. The increase of [Ca^<2+>]i of hepatocytes in physiological condition indicate the direct injury of halothane to hepatocyte cell membrane.