Simple and Rapid Identification of the Mutans Group of Streptococci by Enzyme Immunoassay using Monoclonal Antibodies

采用酶免疫法（EIA）检测了5种不同特异性的单克隆抗体与口腔细菌参比菌的反应性。单克隆抗体a-21、b3-22、f-89、53 -9、h-448分别与a血清型蟋蟀、b血清型鼠、c、e、f血清型变形链球菌、e血清型变形链球菌和d、g血清型sobrinus发生特异性反应。结果表明，临床分离的链球菌具有不同的生化特性，与传统的生化反应方法相比，酶免疫分析法中的单克隆抗体可用于快速、简便地鉴定突变型链球菌。综上所述，单克隆抗体f-89的酶免疫测定系统经过一些改进，已成为一种非常有用的龋活性检测方法。将免疫小鼠脾细胞与小鼠骨髓瘤细胞（Sp2/0-Ag14）融合制备单克隆抗体（MAb f-77）。用从急性肾小球肾炎患者身上分离的变形链球菌（c、e、f血清型）全细胞、参考菌株（STCC 19615）全细胞和几株化脓性链球菌进行酶免疫测定，获得单克隆抗体。利用各种半抗原进行的竞争性酶免疫分析表明，n -乙酰氨基葡萄糖完全抑制了MAb f-77与s.p ogenes细胞之间的反应。免疫电镜还显示，在这些链球菌的细胞表面观察到免疫金颗粒。13EA05：这些结果表明变形链球菌和化脓性链球菌具有一个具有n -乙酰氨基葡萄糖表位的抗原结构。变形链球菌可能在人体内产生与剂量化脓性链球菌相同的免疫反应，并在某些疾病过程中发挥重要作用。

Five monoclonal antibodies (MAbs) with defferent specificity were tested in enzyme immunoassay (EIA) for their reactivity with reference strains of oral bacteria. The MAbs, a-21, b3-22, f-89, s3-9, h-448 reacted specifically with S.cricetus (serotype a), S.rattus (serotype b), S.mutans (serotypes c, e and f), S.mutans (serotype e), and S.sobrinus (serotypes d and g), restectively.The results indicate a diversity of biochemical properties in clinical isolates and suggest that the MAbs in enzyme immunoassay be useful for rapid and simple identification of the mutans group of streptococci, as compared with the conventional method using biochemical reactions.Based on the results above mentioned the enzyme immunoassay system with monoclonal antibody f-89 was considered to become, after some improvement, very useful as a caries activity test.A monoclonal antibody (MAb f-77) was prepared by fusing spleen cells of immune mice and mouse myeloma cells (Sp2/0-Ag14). The monoclonal antibody reaced in enzyme immunoassay with whole cells of Streptococcus mutans strains (serotypes c, e and f) and whole cells of a reference strain (STCC 19615) and several isolates of Streptococcus pyogenes from patients with acute glomerulonephritis. It was demonstrated in competitive enzyme immunoassay using various haptens that reactions between the MAb f-77 and S.pyogenes cells were completely inhibited by N-acetylglucosamine. It was also shown by immunoelectron microscopy that immunogold particles were observed on the cell surfaces of these streptococcal species.13EA05 : These results indicate that S.mutans and S.pyogenes share an antigen structure with an epitope of N-acetylglucosamine. It is possible that S.mutans may produce the same immune response in humans as dose S.pyogenes and play an important role in some disease processes.