Simple and Rapid Identification of the Mutans Group of Streptococci by Enzyme Immunoassay using Monoclonal Antibodies

用酶免疫分析法(EIA)检测了5株特异性不同的单抗与口腔细菌参比菌株的反应性。这些单抗a-21、b3-22、f-89、S3-9、h-448分别与链球菌(a型)、鼠链球菌(b型)、变形链球菌(c、e和f型)、变形链球菌(e型)和远缘链球菌(d和g型)发生特异性反应。基于上述结果，经改进后的单抗f-酶免疫分析系统是一种非常有用的龋病活动性检测方法。用c、e、f三种血清型的变形链球菌全细胞和标准菌株(STCC 19615)的全细胞，以及几株急性肾炎患者分离的化脓性链球菌，用该单抗进行了酶免疫分析。用多种半抗原竞争酶免疫分析表明，N-乙酰氨基葡萄糖可完全抑制单抗f-77与化脓性链球菌的反应。免疫电子显微镜还显示，在这些链球菌的细胞表面观察到免疫金颗粒。13EA05：这些结果表明变形链球菌和化脓性链球菌具有N-乙酰氨基葡萄糖表位的抗原结构。变形链球菌在人体内可能产生与化脓性链球菌相同的免疫应答，并在某些疾病过程中发挥重要作用。

Five monoclonal antibodies (MAbs) with defferent specificity were tested in enzyme immunoassay (EIA) for their reactivity with reference strains of oral bacteria. The MAbs, a-21, b3-22, f-89, s3-9, h-448 reacted specifically with S.cricetus (serotype a), S.rattus (serotype b), S.mutans (serotypes c, e and f), S.mutans (serotype e), and S.sobrinus (serotypes d and g), restectively.The results indicate a diversity of biochemical properties in clinical isolates and suggest that the MAbs in enzyme immunoassay be useful for rapid and simple identification of the mutans group of streptococci, as compared with the conventional method using biochemical reactions.Based on the results above mentioned the enzyme immunoassay system with monoclonal antibody f-89 was considered to become, after some improvement, very useful as a caries activity test.A monoclonal antibody (MAb f-77) was prepared by fusing spleen cells of immune mice and mouse myeloma cells (Sp2/0-Ag14). The monoclonal antibody reaced in enzyme immunoassay with whole cells of Streptococcus mutans strains (serotypes c, e and f) and whole cells of a reference strain (STCC 19615) and several isolates of Streptococcus pyogenes from patients with acute glomerulonephritis. It was demonstrated in competitive enzyme immunoassay using various haptens that reactions between the MAb f-77 and S.pyogenes cells were completely inhibited by N-acetylglucosamine. It was also shown by immunoelectron microscopy that immunogold particles were observed on the cell surfaces of these streptococcal species.13EA05 : These results indicate that S.mutans and S.pyogenes share an antigen structure with an epitope of N-acetylglucosamine. It is possible that S.mutans may produce the same immune response in humans as dose S.pyogenes and play an important role in some disease processes.