Molecular and Pathological Analysis of Anticoagulant Heparan Sulfate Proteoglycan from Endothelial Cell

内皮细胞抗凝硫酸乙酰肝素蛋白多糖的分子和病​​理学分析

• 批准号: 04671519
• 负责人: TAKAMATSU Junki
• 金额: $ 0.38万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671519/
• 关键词: Heparan Sulfate; Proteoglycan; Ryudocan; cDNA; Chromosomal Location; プロテオグリカン; 1型膜蛋白

We have isolated a series of overlapping cDNA clones encoding a 2,610 bp transcript, which potentially codes for a 198 amino acid protein with predicted molecular mass of 21,641 daltons, for the human ryudocan core protein. The deduced core proteins of the human and the rat ryudocan have high structural conservation, particularly in the NH_2 and COOH terminus regions of the putative mature core protein, including the combined transmembrane/cytoplasmic domains with conserved positions of all 4 tyrosine groups and 3 conserved glycosaminoglycan chain attachment regions, which might serve important roles for biological function of ryudocan. A major 2.7 kb transcript was detected in all tissues tested, with relatively high levels of expression observed in mRNA from lung, liver, skeletal muscle and kidney. A minor 1.9 kb transcript was also observed in some of tissues, which would be caused by alternative polyadenylation. Human ryudocan gene has been lacalized on the chromosome 20q12 by fluorescence in situ hybridization. Isolation of the human ryudocan core protein cDNA will allow us to study for the regulation of ryudocan expression and role of this molecule in human endothelial cell function.

我们已经分离出一系列重叠的cDNA克隆，编码2610 bp的转录本，该转录本可能编码198个氨基酸，预测分子质量为21641道尔顿，用于人类鱼多酶核心蛋白。推断的人和大鼠鱼多聚糖核心蛋白具有高度的结构保守性，特别是在假定的成熟核心蛋白的NH_2和COOH端，包括所有4个酪氨酸基团和3个糖胺聚糖链连接区域的跨膜/胞质结合结构域，这些结构域的保守位置可能对鱼多聚糖的生物学功能起重要作用。在所有测试组织中检测到一个主要的2.7 kb转录本，在肺、肝脏、骨骼肌和肾脏的mRNA中观察到相对较高的表达水平。在一些组织中还观察到一个1.9 kb的小转录本，这可能是由选择性聚腺苷酸化引起的。利用荧光原位杂交技术，在20q12染色体上定位到了人琉球酶基因。人琉球蛋白核心蛋白cDNA的分离将为研究琉球蛋白表达调控及其在人内皮细胞功能中的作用奠定基础。

项目成果

Junki Takamatsu et al.: "Human Ryudocan Core Protein : Molecular Cloning and Characterization of the cDNA, and Chromosomal Localization of the Gene" Biochem.Biophys.Res.Commun.190. 814-822 (1993)

Junki Takamatsu 等人：“人类 Ryudocan 核心蛋白：cDNA 的分子克隆和表征，以及基因的染色体定位”Biochem.Biophys.Res.Commun.190。

Junki Takamatsu ほか: "Human Ryudocan Core Protein:Molecular Cloning and Characterization of the cDNA,and Chromosomal Localization of the Gene." Biochem.Biophys.Res.Commun.190. 814-822 (1993)

Junki Takamatsu 等人：“人类 Ryudocan 核心蛋白：cDNA 的分子克隆和表征，以及基因的染色体定位。”Biochem.Biophys.Res.Commun.190（1993）。

Junki Takamatsu et al.: "Human Ryudocan Core Protein:Molecular Cloning and Characterization of the cDNA,and Chromosomal Localization of the Gene" Biochem.Biophys.Res.Commun.190. 814-822 (1993)

Junki Takamatsu 等人：“人类 Ryudocan 核心蛋白：cDNA 的分子克隆和表征，以及基因的染色体定位”Biochem.Biophys.Res.Commun.190。