Molecular and Pathological Analysis of Anticoagulant Heparan Sulfate Proteoglycan from Endothelial Cell

我们已经分离了一系列重叠的cDNA克隆，其编码一个2,610 bp的转录本，该转录本可能编码一个198个氨基酸的蛋白质，预测分子量为21,641道尔顿，用于人ryudocan核心蛋白。推测的人和大鼠ryudocan的核心蛋白具有高度的结构保守性，特别是在推测的成熟核心蛋白的NH_2和COOH末端区域，包括跨膜/胞质结合结构域和3个保守的糖胺聚糖链连接区，它们可能对ryudocan的生物学功能具有重要作用。一个主要的2.7 kb的转录检测在所有组织测试，与相对较高的表达水平，从肺，肝，骨骼肌和肾的mRNA中观察到。在某些组织中也观察到一个较小的1.9kb转录本，这可能是由选择性多聚腺苷酸化引起的。用荧光原位杂交技术将人ryudocan基因定位于染色体20 q12。人ryudocan核心蛋白cDNA的分离将使我们能够研究ryudocan表达的调控以及该分子在人内皮细胞功能中的作用。

We have isolated a series of overlapping cDNA clones encoding a 2,610 bp transcript, which potentially codes for a 198 amino acid protein with predicted molecular mass of 21,641 daltons, for the human ryudocan core protein. The deduced core proteins of the human and the rat ryudocan have high structural conservation, particularly in the NH_2 and COOH terminus regions of the putative mature core protein, including the combined transmembrane/cytoplasmic domains with conserved positions of all 4 tyrosine groups and 3 conserved glycosaminoglycan chain attachment regions, which might serve important roles for biological function of ryudocan. A major 2.7 kb transcript was detected in all tissues tested, with relatively high levels of expression observed in mRNA from lung, liver, skeletal muscle and kidney. A minor 1.9 kb transcript was also observed in some of tissues, which would be caused by alternative polyadenylation. Human ryudocan gene has been lacalized on the chromosome 20q12 by fluorescence in situ hybridization. Isolation of the human ryudocan core protein cDNA will allow us to study for the regulation of ryudocan expression and role of this molecule in human endothelial cell function.