Research on the pyrenoid in Chlamydomonas

衣藻类蛋白核的研究

• 批准号: 05804047
• 负责人: TSUZUKI Mikio
• 金额: $ 1.15万
• 依托单位: Tokyo University of Pharmacy and Life Science (1994-1995)The University of Tokyo (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05804047/
• 关键词: pyrenoid; carbon dioxide; microalgae; Chlamydomonas; Rubisco; starch

The pyrenoid, which is located in chloroplast of algae, increases under low CO_2 conditions by about 3 times in Chlamydomonas. Sucrose density gradient centrifugation of the pyrenoid fixed with HgC1_2 showed relatively heavier pyrenoid in the cells adapted to low CO_2 condition. Average protein content of the pyrenoid showed the same result. During the adaptation to low CO_2 concentration where the pyrenoid developed, rbcS1, one of two genes of small subunit of Rubisco, was transcribed less than that in high CO_2 condition, while rbcS2, the other Rubisco gene, was at almost the same level. The development of the pyrenoid was still observed in the presence of chloramphenicol.

类蛋白核位于藻类叶绿体中，在低CO_2条件下，衣藻体内的蛋白核含量增加了约3倍。用HgCl2固定的类蛋白核在蔗糖密度梯度离心法中显示，在适应低CO2条件的细胞中类蛋白核相对较重。类蛋白核的平均蛋白质含量也显示出相同的结果。在对类蛋白核形成的低CO_2浓度的适应过程中，Rubisco小亚基的两个基因之一rbcS1的转录水平低于高CO_2条件下的转录水平，而另一个Rubisco基因rbcS2的转录水平与高CO_2条件下几乎相同。在氯霉素存在的情况下，仍能观察到类蛋白核的发育。

项目成果

N.Sato,K.Sonoike,A.Kawaguchi,& M.Tsuzuki: "Contribution of lopvered unsaturation levels of chloroplast lipids to high temperature tolerance of photosynthesis in chlamy domonas reinhardtii." J.Photochem. Photobiol.(in press). (1996)

N.佐藤，K.园池，A.川口，

M.Tsutsumi, K.Kawachi, M.Kaneko, and M.Tsuzuki: CO2 fixation system by Chlorella on a filter membrane. In Photosynthesis : from Light to Bioshere. Vol. V, p. 881-884. Edited by P.Mathis. Kluwer academic Phubishers, the Netherlands, (1995)

M.Tsutsumi、K.Kawachi、M.Kaneko 和 M.Tsuzuki：小球藻在滤膜上的 CO2 固定系统。

N.Sato, A.Kawaguchi, and M.Tsuzuki: "Contribution of lowered unsaturation levels of chloroplast lipids to hith temperature tolerance of photosynthesis in Chlamydomonas reinhardtii" J.Photochem. Photobiol.(in press).

N.Sato、A.Kawaguchi 和 M.Tsuzuki：“降低叶绿体脂质不饱和水平对莱茵衣藻光合作用高温耐受性的贡献”J.Photochem。

N. Sato, K. Sonoike, A. Kawaguchi, & M. Tsuzuki: "Contribution of lowered unsaturation lerels of chloroplast 1ipids to high temperature tolerance of photosynthesis in Chlamydononas reinhardtii." J.Photochem. Photobiol.(in press). (1996)

N.佐藤、K.园池、A.川口、

M.Tsutsumi,K.Kawachi,M.Kaneko,& M.Tsuzuki: "CO_2 tixation system by Chlorella on a filter membrane." In Photosynthesis:from Light to Biosphere. Edited by P.Mathis. Kluwer Acad. Pabl.vol.V. 881-884 (1995)

M.Ttsutsumi、K.Kawachi、M.Kaneko、