Development of the concentration and separation system for multi-compornents by ion-pair phase separation phenomena with fluorosurfactant

通过全氟辛酸根离子的电荷中和，发现了两种新的三相分离现象；在本研究中，根据从这些相分离现象获得的基本发现，详细讨论了几种金属螯合物和生理活性底物的浓缩和分离。在胺型(例如，在存在正戊胺的情况下)，在pH值为7~8的范围内，证实了三相分离现象，每一相依次为水相和两种不溶水相(第二相和第一相)。利用这一现象，将均相液-液萃取法应用于L-抗坏血酸、血色素和α，β，γ，β-四(4-三甲氨基苯基)卟啉三组分的分离，以分离人…中的药用物质结果表明，只需在全氟辛酸溶液中加入淀粉胺，即可将它们分离为水(L-抗坏血酸)、第二相(血红蛋白)和第一相(TTMAPP)。另一方面，在极性有机溶剂型(如有二甲基亚砜存在的情况下)，在pH值为0.42~0.46的范围内，证实了三相分离现象。分别在水相、第二相和第一相中定量提取和分离了三种水溶性的卟啉。这些溶质的结构和物理化学性质是将其应用于其他溶液分离的重要模型。综上所述，在过去的两年中，上述结果对开发新的分离方法“多组分均相液-液萃取”取得了有价值的结果。在不久的将来，新的多相分离现象的发现和在含有有用溶质的大范围样品中的应用将是积极的。较少

New two types of three-phase separation phenomena were found by the charge neutralization of perfluorooctanate ion ; they are amine type in the neutral pH to alkaline solution and that of polar organic solvent in the acidic solution.In this study, the concentration and separation of several kinds of metal-chelate and physiological active substrates have been discussed in detail based on fundamental findings obtained from these phase separation phenomena.In the amine type (e.g.the case in the presence of n-amylamine), threephase separation phenomenon was confirmed in the pH range of 7 to 8.Each phases were successively water phase and two kinds of water-immisible phase (second phase and first phase) from upper side.The homogeneous liquid-liquid extraction method using these phenomena was applied to three components such as L-ascorbic acid, hemogrobin and alpha, beta, gamma, delta-tetrakis (4-trimethylaminophenyl) porphine (TTMAPP) assuming that separate the medical substances in human b … More lood.As the results, they were able to be separated into water (L-ascorbic acid), second (hemoglobin) and first (TTMAPP) phases, respectively using only a one-step process with addition of amylamine to the perfluorooctanoic acid solution.On the other hand, in the polar organic solvent type (e.g.the case in the presence of dimethylsulfoxide), the three-phase separation phenomenon was confirmed in the pH range of 0.42 to 0.46.This extraction method was applied to eight kinds of synthetic water-soluble porphyrin.As the results, three kinds of water-soluble porphyrin was quantitatively extracted and separated in water phase, second phase and first phase, respectively.The structure and physico-chemical property of these solutes is an important as a model when this extraction method is applied to the separation for other solutes.In conclusion, above-described valuable results for the development of new separation method "multi-compornents homogeneous liquid-liquid extraction"was obtained during the past two years.In the near future, the discovery of novel multi-phase separation phenomenon and the application to the extensive sample containing useful solute would be aggressively done. Less