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DEVELOPMENT OF AN ELECTROCHEMICAL MEASUREMENT METHODS ABOUT BIOFUNCTIONAL MATERIALS AND THEIR APPLICATION TO NONAQUEOUS SOLUTION SYSTEMS

生物功能材料电化学测量方法的开发及其在非水溶液体系中的应用

基本信息

批准号：
06640778
负责人：
NAKAMURA Toshio
金额：
$ 1.28万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Studies for getting basic data concerning to redox and electron transfer about biofunctional materials and for developing sensors for use both in detection of those and in tracing their activities were carried out. Biofunctional materials, which are generally difficuit to exchange electrons between those and an electrode, were contained in fairly a thin film of hydrophilic polymer, polyacrylamide (PAA)coated on a platinum disk electrode. A cell was constructed with the electrode and an acentonitrile(AN) solution to investigate electrochemical functions. 1)A PAA and methylviologen were dissolved with water on a platinum disk and dried in a sil-ica gel descicator. From the cyclic voltammetry (CV) the cell constructed with the electrode was confirmed to share a necessary condition for carring out the research. 2)The platinum disk electrodes coated with a PAA membrane containing a heme protein (catalase, bleomycin, hemoglobin, or cytochrome c) were constructed. It was found from the electrode that CV behavior about the redox of oxygen were different each other according to a containing protain. It should be nescessary to investigate the effect against the fedox behavior of oxygen by the difference of protains and the charge of the iron. 3)The platinum disk electrodes coated with a PAA membrane con-taining some functional materials(NADH,NAD,PQQ,diaphrase)were constructed. It was found from the electrode that redox peak currents were proportional to the con-centration of the cyclohexanol in AN.4)The PAA in gel type used in the experiment as a matrix for fixing biofunctional materials close to platinum disk was investi-gated to elucidate electrochemical basic characteristics.(Pacifichem'95, part 1, ANYL 193(1995). (Honolulu, Dec 17-22))Informations about redox behavior of a cho-lesterol and an oxygen monoxide were also obtained by the platinum electrode coated with a PAA containing cobalt phthalocyanine or hemoglobine.

开展了关于生物功能材料氧化还原和电子转移的基本数据的研究，以及开发用于检测和跟踪其活性的传感器的研究。生物功能材料通常很难在这些材料和电极之间交换电子，它们被包含在一层相当薄的亲水性聚合物中，聚丙烯酰胺(PAA)涂在铂盘电极上。用该电极和丙烯腈(AN)溶液构建了电池，以研究其电化学功能。1)将PAA和甲基紫精在铂盘上用水溶解，并在Sili-ICA凝胶降解器中干燥。从循环伏安法(CV)证实，用该电极构建的电池具有开展研究的必要条件。2)在铂电极表面涂覆一层含有血红素蛋白(过氧化氢酶、博莱霉素、血红蛋白或细胞色素c)的PAA膜。从电极上发现，氧的氧化还原的循环伏安行为因所含蛋白质的不同而不同。因此，有必要研究不同浓度的铁和铁的电荷对氧的氧化还原行为的影响。3)在铂电极表面涂覆一层含有某些功能材料(NADH、NAD、PQQ、隔膜)的PAA膜。从电极上发现，氧化还原峰电流与环己醇的浓度成正比。4)以凝胶型PAA为基质，将生物功能材料固定在铂圆盘附近，以阐明其电化学基本特性(太平洋95，第1部分，ANYL 193(1995))。(火奴鲁鲁，12月17日至22日))通过在铂电极上涂覆含钴酞菁或血红素的PAA，还获得了有关胆甾醇和氧一氧化碳氧化还原行为的信息。