DEVELOPMENT OF AN ELECTROCHEMICAL MEASUREMENT METHODS ABOUT BIOFUNCTIONAL MATERIALS AND THEIR APPLICATION TO NONAQUEOUS SOLUTION SYSTEMS

为获得生物功能材料氧化还原和电子转移的基本数据，开发用于检测和跟踪其活性的传感器进行了研究。生物功能材料通常很难在它们和电极之间交换电子，它们被包含在相当薄的亲水聚合物薄膜中，聚丙烯酰胺（PAA）涂在铂盘电极上。用该电极和乙腈（an）溶液构建电池，研究其电化学功能。1) PAA和甲基紫素在铂盘上用水溶解，在硅胶干燥器中干燥。循环伏安法（CV）证实了用该电极构建的电池具有开展研究的必要条件。2)构建包被含有血红素蛋白（过氧化氢酶、博来霉素、血红蛋白或细胞色素c）的PAA膜的铂盘电极。从电极上发现，氧氧化还原的CV行为根据所含蛋白质的不同而不同。因此，有必要研究蛋白质的不同和铁的电荷对氧氧化还原行为的影响。3)构建了包被含有功能材料（NADH、NAD、PQQ、膜片酶）的PAA膜的铂盘电极。从电极上发现，氧化还原峰值电流与环己醇在an中的浓度成正比。4)实验中采用凝胶型PAA作为基质，将生物功能材料固定在靠近铂盘的地方，以阐明其电化学基本特性。（Pacifichem'95, part 1, ANYL 193(1995)）。（檀火奴鲁鲁，12月17-22日）)通过铂电极涂覆含有酞菁钴或血红蛋白的PAA，也获得了胆固醇和一氧化碳氧化还原行为的信息。

Studies for getting basic data concerning to redox and electron transfer about biofunctional materials and for developing sensors for use both in detection of those and in tracing their activities were carried out. Biofunctional materials, which are generally difficuit to exchange electrons between those and an electrode, were contained in fairly a thin film of hydrophilic polymer, polyacrylamide (PAA)coated on a platinum disk electrode. A cell was constructed with the electrode and an acentonitrile(AN) solution to investigate electrochemical functions. 1)A PAA and methylviologen were dissolved with water on a platinum disk and dried in a sil-ica gel descicator. From the cyclic voltammetry (CV) the cell constructed with the electrode was confirmed to share a necessary condition for carring out the research. 2)The platinum disk electrodes coated with a PAA membrane containing a heme protein (catalase, bleomycin, hemoglobin, or cytochrome c) were constructed. It was found from the electrode that CV behavior about the redox of oxygen were different each other according to a containing protain. It should be nescessary to investigate the effect against the fedox behavior of oxygen by the difference of protains and the charge of the iron. 3)The platinum disk electrodes coated with a PAA membrane con-taining some functional materials(NADH,NAD,PQQ,diaphrase)were constructed. It was found from the electrode that redox peak currents were proportional to the con-centration of the cyclohexanol in AN.4)The PAA in gel type used in the experiment as a matrix for fixing biofunctional materials close to platinum disk was investi-gated to elucidate electrochemical basic characteristics.(Pacifichem'95, part 1, ANYL 193(1995). (Honolulu, Dec 17-22))Informations about redox behavior of a cho-lesterol and an oxygen monoxide were also obtained by the platinum electrode coated with a PAA containing cobalt phthalocyanine or hemoglobine.