The behavior of the trans-Golgi networks in plant cells.

利用冷冻置换法和免疫电镜技术研究了植物细胞中作为囊泡运输分选位点的反式高尔基体网络（trans-Golgi networks，TGNs）的行为。（红藻纲）、臂单胞菌属、Trebouxia属、Ankistrodesmus属、盘星藻属、栅藻属和葡萄球菌属本文以紫露草属（Tradescantia）植物（绿藻纲）、真藓属（Bryum）、花粉和花粉管为研究对象，对高等植物组织细胞中已知的TGN进行了研究。除紫露草属的花粉管外，其它所有细胞中都能观察到TGN。因此，TGN在从红藻到高等植物的细胞中普遍存在，葡萄藻属中的TGN结构最大，因此，本研究对葡萄藻属中TGN的结构和行为进行了系统的研究。网格蛋白包被的囊泡总是附着在TGN上。TGN在细胞分裂过程中形成小泡，参与隔膜的形成;在细胞分裂后形成多泡体，参与液泡的形成。由高尔基体衍生的囊泡似乎是通过TGN运输到它们的指定位点的，我们先后产生了抗高尔基体-TGN和抗内质网的抗体。我们将这些抗体应用于免疫电子显微镜，以检查与TGN相关的蛋白质分子的运动。

The behavior of the trans-Golgi networks (TGNs) in plant cells which are the sorting site of vesicle transport were examined by freeze substitution method and immuno-electron microscopy.The existence and the structure of TGNs in Cyanidium (Rhodophyceae) , Brachiomonas, Trebouxia, Ankistrodesmus, Pediastrum, Scenedesmus and Botryo-coccus (Chlorophyceae) , Bryum (Moss) , pollens and pollen tubes of Tradescantia (higher plant) were studied to get information about the TGNs which have little known in the tissue cells of higher plants. In all cells except for the pollen tubes of Tradescantia, TGNs could be observed. Accordingly, it can be suggested that the universal occurrence of the TGNs in cells from Rodophyceae to higher plants.The TGNs in Botryococcus was the biggest in structure among the cells listed above, so that the structure and the behavior of the TGN in Botryococcus were studied in ditail throughout the cell cycle. Clathrin-coated vesicles were always attached to the TGNs. The TGNs formed the vesicles which participated in the septum formation during cell division and produced multi-vesicular bodies which participated in the vacuole formation after cell division. Vesicles derived from Golgi bodies seemed to be transported to their distination sites via TGNs.The antibodies against to the Golgi-TGN and to the endoplasmic reticulum were successively produced by us. We are applying these antibodies to the immuno-electron microscopy to examine the movement of protein molecules associated TGNs.