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The behavior of the trans-Golgi networks in plant cells.

植物细胞中跨高尔基体网络的行为。

基本信息

批准号：
06640864
负责人：
NOGUCHI Tetsuko
金额：
$ 1.47万
依托单位：
NARA WOMEN'S UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The behavior of the trans-Golgi networks (TGNs) in plant cells which are the sorting site of vesicle transport were examined by freeze substitution method and immuno-electron microscopy.The existence and the structure of TGNs in Cyanidium (Rhodophyceae) , Brachiomonas, Trebouxia, Ankistrodesmus, Pediastrum, Scenedesmus and Botryo-coccus (Chlorophyceae) , Bryum (Moss) , pollens and pollen tubes of Tradescantia (higher plant) were studied to get information about the TGNs which have little known in the tissue cells of higher plants. In all cells except for the pollen tubes of Tradescantia, TGNs could be observed. Accordingly, it can be suggested that the universal occurrence of the TGNs in cells from Rodophyceae to higher plants.The TGNs in Botryococcus was the biggest in structure among the cells listed above, so that the structure and the behavior of the TGN in Botryococcus were studied in ditail throughout the cell cycle. Clathrin-coated vesicles were always attached to the TGNs. The TGNs formed the vesicles which participated in the septum formation during cell division and produced multi-vesicular bodies which participated in the vacuole formation after cell division. Vesicles derived from Golgi bodies seemed to be transported to their distination sites via TGNs.The antibodies against to the Golgi-TGN and to the endoplasmic reticulum were successively produced by us. We are applying these antibodies to the immuno-electron microscopy to examine the movement of protein molecules associated TGNs.

采用冷冻替代法和免疫电镜技术研究了植物细胞中作为囊泡运输分选位点的跨高尔基体网络（TGN）的行为。蓝藻（红藻）、腕单胞菌、Trebouxia、Ankistrodesmus、Pediastrum、Scenedesmus和Botryo-coccus中TGN的存在和结构对绿藻纲（Chlorophyceae）、苔藓纲（Bryum）、紫露草（Tradescantia）（高等植物）的花粉和花粉管进行了研究，以获得有关高等植物组织细胞中鲜为人知的TGN的信息。在除紫露草花粉管外的所有细胞中，都可以观察到 TGN。因此，可以表明TGN在从红藻纲到高等植物的细胞中普遍存在。在上述列出的细胞中，葡萄球菌中的TGN在结构上是最大的，因此在整个细胞周期中对葡萄球菌中TGN的结构和行为进行了详细研究。网格蛋白包被的囊泡总是附着在 TGN 上。 TGNs形成囊泡，参与细胞分裂过程中隔膜的形成，并产生多囊泡体，参与细胞分裂后液泡的形成。来自高尔基体的囊泡似乎是通过TGNs转运到目的地的。我们先后制备了针对高尔基体-TGN和内质网的抗体。我们正在将这些抗体应用于免疫电子显微镜，以检查与 TGN 相关的蛋白质分子的运动。