Non-protein amino acids reflect the phylogeny of living organisms? -in the case of the genus Amanita (Basidiomycotina).

非蛋白质氨基酸反映了生物体的系统发育？

• 批准号: 06640908
• 负责人: HATANAKA Shin-ichi
• 金额: $ 1.34万
• 依托单位: International Cristian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640908/
• 关键词: Amanita gymnopus; Amanita hongoi; Amanita abrupta; Amanita; Roanokenses; (2S)-2-Amino-5-chloro-4-hydroxy-5-hexenoic acid; (2S)-2-Amino-5-chloro-6-hydroxy-4-hexenoic acid; (2S)-2-Amino-5-chloro-4-hydroxy-5-hexenoic acid; A. hongoi; A. abrupta; 2S

Most of the fungi belonging the genus Amanita, subgenus Lepidella, section Roanokenses accumulate species-specific non-protein amino acids. The aim of this research project was to ascertain this phenomenon in more kinds of fungal species and to investigate, together with the morphological consideration, if non-protein amino acids-both kinds and their distribution pattern-reflect the phylogenetical relationships within the taxon of fungi.Although we could analyze for two years only three kind of fungi, species-specificity of the amino acids has greatly strengthen. Furthermore, two closely related fungi, i.e. Amanita abrupta and A.hongoicontain the same specific chlorine-containing (2S)-2-amino-5-choloro-6-hydroxy-4-hexenoic acid. Fruitbodies of these two fungi differ each other greatly in appearance. Such analytical data could be possible to be utilize for the identification of fungi which are not easy to distinguish from each other by morphological characters.The fungi analyzed in the first year in the present project had been identified with a very closely species to A.cokeri (Gilb.et Kuhn) Gilb.f.roseotincta Nagasawa & Hongo. Later precisc morphological investigation, however, revealed that it is reasonable to consider a new species. Manuscript for it is now in preparing.

属于鹅膏菌属（Amanita）、乳鹅膏菌亚属（Subgenus Lampelella）、Roanokenses组（Section Roanokenses）的大多数真菌积累种特异性非蛋白质氨基酸。本研究的目的是在更多种类的真菌中确定这种现象，并结合形态学的考虑，调查非蛋白质氨基酸的种类及其分布模式是否反映了真菌分类群内的亲缘关系。虽然我们只能分析三种真菌，但两年来，氨基酸的种特异性大大加强。此外，两种密切相关的真菌，即白鹅膏菌和红鹅膏菌含有相同的特定含氯（2S）-2-氨基-5-氯-6-羟基-4-己烯酸。这两种真菌的子实体在外观上有很大差异。本研究第一年分析的真菌与A.cokeri（Gilb. et Kuhn）Gilb.f.roseotincta Nagasawa & Hongo的种关系非常密切。然而，后来的精确形态学研究表明，考虑一个新种是合理的。目前，这方面的工作正在准备之中。

项目成果

Hatanaka, S.I., Okada, K.and Nagasawa, E.: "Isolation and identification of (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid from an Amanita of the section Roanokenses (Amanitaceae)" Mycoscience. Volume 36. 391-394 (1995)

Hatanaka, S.I.、Okada, K. 和 Nagasawa, E.：“从伞形毒伞科（鹅膏科）伞形毒蕈中分离和鉴定 (2S)-2-氨基-5-氯-4-羟基-5-己烯酸”

Kudo, S.and Nagasawa, E.: "A poisoning case by Lepista graveolens in Japan" Nippon Kingakukai Kaihou. Volume 36. 103-106 (1995)

Kudo, S. 和 Nagasawa, E.：“日本 Lepistagravolens 中毒事件”Nippon Kingakukai Kaihou。

畑中信一.岡田久美子.長沢栄史: "Isolation and identitication of (2S)-2-amino-5-chloro-4-hydroxy-5-hexenoic acid from an Amanita of the section Ruanokenses (Amenitaceae)" Mycoscience. 36. 395-397 (1995)

Shinichi Hatanaka. Kumiko Okada. Eishi Nagasawa：“从伞形科鹅膏菌中分离和鉴定 (2S)-2-氨基-5-氯-4-羟基-5-己烯酸”Mycoscience 36。 -397 (1995)

長沢栄史: "A new species of Boletus sect.Boletus from Japan." 日本学士院紀要. 70B. 10-14 (1994)

Eishi Nagasawa：“牛肝菌属新种。来自日本的牛肝菌。”70B 10-14（1994）。

Hatanaka S.I.,Furukawa J.,Aoki T.,Akatsuka H.and Nagasawa E.: "(2S)-2-Amino-5-chloro-4-hydroxy-5-hexenoic acid,and related comounds from Amanita gymnopus." Mycoscience. 35. 391-394 (1994)

Hatanaka S.I.、Furukawa J.、Aoki T.、Akatsuka H. 和 Nagasawa E.：“(2S)-2-氨基-5-氯-4-羟基-5-己烯酸，以及来自鹅膏菌的相关化合物。”