Mapping resistant genes for green rice leafhopper (Nephotettix cincticeps UHLER) using molecular markerrs in rice

利用水稻分子标记绘制绿稻叶蝉 (Nephotettix cincticeps UHLER) 的抗性基因图谱

• 批准号: 06660010
• 负责人: YASUI Hideshi
• 金额: $ 1.34万
• 依托单位: Kyushun University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660010/
• 关键词: resistance to green rice leafhopper (Nephotettix cincticeps UHLER); near isogenic lines; RFLP analysis; DNA marker; recombinant inbred lines; complementary genes; タイワンツマグロヨコバイ; ツマグロヨコバイ; Oryza sativa L.; 抵抗性遺伝子; トリソミック分析; 組換自殖系統

Genetic analysis was carried out to determine the locus of resistance to green rice leafhopper (GRLH) (Nephotettix cincticeps UHLER). Near isogenic lines with GRLH resistant genes were developed by four or three backcrossing using Kinmaze or Taichung 65 as recurrent parents and DV 85 as a donor parent. Genetic segregation ratios in the three F2 populations indicated two dominant complementary genes govern GRLH resistance of DV 85. RFLP analysis revealed that one of the resistant genes was tightly linked with RFLP marker, Xnpb144 on chromosome 3, and that the other resistant gene was linked with RFLP markers, Xnpb181 and G1465 on chromosome 11 in one of the F2 populations. Among two RFLP markers linked to GRLH resistance on chromosome 11, G1465 was most tightly linked to the resistant gene.Additionally, Genetic analysis was carried out by using recombinant inbred lines derived from a cross between japonica variety Kinmaze and indica variety DV 85. The genetic segregation ratios of resistance to susceptible was fitted to 1 : 3 indicating two dominant complementary genes conferring the GRLH resistance.

对水稻绿叶蝉(NePhoettix Cincticep Uhler)进行了遗传分析，确定了其抗性基因。以金迷子或台中65为轮回亲本，DV 85为供体亲本，通过4~3次回交，获得了具有GRLH抗性基因的近等基因系。三个F_2群体的遗传分离比表明，两个显性互补基因控制DV-85对GRLH的抗性。RFLP分析表明，其中一个抗性基因与第3染色体上的RFLP标记Xnpb144紧密连锁，另一个抗性基因与第11染色体上的RFLP标记Xnpb181和G1465紧密连锁。在11号染色体上与GRLH抗性连锁的两个RFLP标记中，G1465与抗性基因连锁最紧密。此外，还利用来自水稻品种金梅泽和水稻品种DV 85的重组自交系进行了遗传分析。抗性与感病的遗传分离比符合1：3，表明有两个显性互补基因控制GRLH抗性。