Mapping resistant genes for green rice leafhopper (Nephotettix cincticeps UHLER) using molecular markerrs in rice

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Genetic analysis was carried out to determine the locus of resistance to green rice leafhopper (GRLH) (Nephotettix cincticeps UHLER). Near isogenic lines with GRLH resistant genes were developed by four or three backcrossing using Kinmaze or Taichung 65 as recurrent parents and DV 85 as a donor parent. Genetic segregation ratios in the three F2 populations indicated two dominant complementary genes govern GRLH resistance of DV 85. RFLP analysis revealed that one of the resistant genes was tightly linked with RFLP marker, Xnpb144 on chromosome 3, and that the other resistant gene was linked with RFLP markers, Xnpb181 and G1465 on chromosome 11 in one of the F2 populations. Among two RFLP markers linked to GRLH resistance on chromosome 11, G1465 was most tightly linked to the resistant gene.Additionally, Genetic analysis was carried out by using recombinant inbred lines derived from a cross between japonica variety Kinmaze and indica variety DV 85. The genetic segregation ratios of resistance to susceptible was fitted to 1 : 3 indicating two dominant complementary genes conferring the GRLH resistance.