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gene expression induced by UV-B Light Study on gene expression induced by UV-B light

gene expression induced by UV-B Light Study on gene expression induced by UV-B light
UV-B 光诱导的基因表达 UV-B 光诱导的基因表达研究
批准号：
06660425
负责人：
TAKEDA Junko
金额：
$1.54万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
项目状态：
已结题
起止时间：
1994 至 1995
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660425/
关键词：
UV-B responsive elementPhenyl alamin ammonia-lyasecanot suspension cellsUV-B responsePAL geneaction spectrumUV-B photoreceptorgene expressionフキニールアラニンアンモニアリアーゼUV-Bプロモータ

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中文摘要
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英文摘要
(1) Carrot pal-10 full length promoter (-2335) fused to reporter gene (luciferase) was transiently transformed to carrot protoplasts by electroporation, and promoter activity (luciferase) induced by UV-B light was examined. Pal-10 full length promotor expression was UV-B specific and involvement of UV-B photoreceptor in regulation of pal-10 gene is satisfied as shown in intact cells.(2) UV-B specific cis element was examined using various length promoter fragments fused to CaMV 35S core promoter (-90). Two elements were obtained between -395 and -190 and their consensus were T (A) XTCXCCAACCC (A).(3) However, truncated promoter at -570, containing UV-B responsive cis-elements did not show UV-B response.(4) Both UV-B responsive cis-element and up (-2335 to -570) and down (-145 to + 145) stream sequences are necessary for correct expression of pal-10 promoter.
期刊论文(16)
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会议论文
Ozeki & Takeda: "Regulation of uhenyl alanine ammoma-lyasl gene in Carret suspension caltwred cells" Plant Cell, Tissue and Organe culture. 38. 221-225 (1994)
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通讯作者：
Ozaki & Takeda J: "Reyulation of plenylalanine ammoms-lyase gene in cenit suspension cuetured cells" Phant Cell. Tissne and organ aulture. 38. 221-225 (1994)
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Takeda J.et al.: "Action spectra of pfenylalanine ammoma-lyase and chalcome synthase expression in carrot cells in suspension" Physial Plant.91. 517-521 (1994)
Takeda J.等人：“悬浮胡萝卜细胞中苯丙氨酸氨合酶和查尔科姆合酶表达的作用谱”Physial Plant.91。
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通讯作者：
Takeda J. et al.: "Action spectra of phenyl alanine ammonia-lyase and chalcone Pynthase expression in cdnt cees in Puspension" Physiologia Plantarum. 91. 517-521 (1994)
Takeda J.等人：“悬浮液中cdnt cees中苯丙氨酸解氨酶和查耳酮平合酶表达的作用谱”Physiologia Plantarum。
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共 8 条
    Nursing Strategies to Promote Young Children's Ability to Cope with Pain
    • 批准号：
      06672317
    • 项目类别：
      Grant-in-Aid for Scientific Research (C)
    • 资助金额：
      $1.28万
    • 财政年份：
      1994
    • 负责人：
      TAKEDA Junko
    • 依托单位：
      Chida University
    Early process on embryogenesis of cultured carrot suspension cells.
    • 批准号：
      61560004
    • 项目类别：
      Grant-in-Aid for General Scientific Research (C)
    • 资助金额：
      $1.6万
    • 财政年份：
      1986
    • 负责人：
      TAKEDA Junko
    • 依托单位：
      Kyoto University