gene expression induced by UV-B Light Study on gene expression induced by UV-B light

(1)通过电穿孔将融合报告基因（荧光素酶）的胡萝卜pal-10全长启动子（-2335）瞬间转化到胡萝卜原生质体中，检测UV-B光诱导下启动子（荧光素酶）的活性。Pal-10全长启动子的表达具有UV-B特异性，并且在完整细胞中满足UV-B光感受器参与Pal-10基因的调控。(2)利用与CaMV 35S核心启动子（-90）融合的不同长度启动子片段检测UV-B特异性顺式元件。在-395和-190之间得到两个元件，它们的一致性是T (A) XTCXCCAACCC (A)。(3)然而，在-570处截断的启动子，含有UV-B响应顺式元件，不表现出UV-B响应。(4) UV-B响应顺式元件和上（-2335 ~ -570）、下（-145 ~ + 145）流序列是pal-10启动子正确表达所必需的。

(1) Carrot pal-10 full length promoter (-2335) fused to reporter gene (luciferase) was transiently transformed to carrot protoplasts by electroporation, and promoter activity (luciferase) induced by UV-B light was examined. Pal-10 full length promotor expression was UV-B specific and involvement of UV-B photoreceptor in regulation of pal-10 gene is satisfied as shown in intact cells.(2) UV-B specific cis element was examined using various length promoter fragments fused to CaMV 35S core promoter (-90). Two elements were obtained between -395 and -190 and their consensus were T (A) XTCXCCAACCC (A).(3) However, truncated promoter at -570, containing UV-B responsive cis-elements did not show UV-B response.(4) Both UV-B responsive cis-element and up (-2335 to -570) and down (-145 to + 145) stream sequences are necessary for correct expression of pal-10 promoter.