Study on the cardiotoxic actions of botulinolysin and other bacterial thiol-activated hemolysins.

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(1) Cardiotoxic action of bacterial thiol-activated hemolysins, rheta-toxin (PLO) of Clostridium perfringens and botulinolysin (BL) of Clostridium botulinum, was investigated by using whole rats, and isolated thier orgains and tissues.(2) Blood pressure of rats abruptly dropped immediately after the i.v.injection of BL.(3) Ex vivo perfusion of BL and PLO to isolated hearts caused irreversible increase in coronary perfusion pressure and cessation of spontaneous heart beat.(4) Increase of perfusion pressure by BL was also observed in lungs, livers, or kidneys.(5) Treatment with BL or PLO increased contractile tension of isolated aortic rings elicited by phenylephrine, alpha-agonist.(6) Tension elicited by phenylephrine in endothelium-denuded rings was not affected by the treatment with BL or PLO.(7) Relaxation of the rings by acetylcholine was inhibited concentration-dependently by BL or PLO.(8) Biochemical confirmation of the actions of BL and PLO on release of EDRF from endothelium was proved to be impossible at the moment because the concentration of NO in perfusate from hearts before and after the perfusion of BL or PLO was too low to be quantified by fluorescent measurement using 2,3-diaminonaphthalene.