Forensic study on death of triazolam intoxication

三唑仑中毒死亡的法医学研究

• 批准号: 06670467
• 负责人: KOMURA Setsuo
• 金额: $ 1.34万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670467/
• 关键词: Triazolam; Deep electroencephalogram; Dying process; エタノール; 血中濃度; 薬物相互作用

In rabbits triazolam gave inhibitory influence to the electroencephalogram (EEG), especially that of the hippocampus and amygdala. The electrocardiogram (ECG) and cortical EEG were also measured.Thirty minutes after 2g/kg ethanol was intraperitoneally administered to rabbits, 5mg/kg triazolam was orally administered are we continuously measured ECG and EEG of cortex and deep area, observing their behavior. Two hours and half after triazolam was administered, blood of ear vein showed 116ng/ml of triazolam by the analysis of gas-chromatography/mass-spectrometry. This concentration is too low to cause the death of rabbits. It is conceivable that fifty times as much as the triazolam concentration is needed to cause the death. More than 4g/kg ethanol may be needed.As for their behavior, about two hours after triazolam was administered on the conditions mentioned above, continuous nystagmus appeared. The cause is still unclear.We also used Wistar male rats and measured the concentration of dopamine and serotonin of the whole brain after the intraperitoneal administration of 2g/kg ethanol. We found no change compared with saline treated group. However the increase of dopamine and serotonin discharge in the nucleus accumbens by the method of brain microdialysis. It is guessed that the same result is obtained in the case of 5mg/kg triazolam administration.

三唑仑对家兔的脑电图有抑制作用，尤其是海马和杏仁核的脑电图。给家兔腹腔注射2g/kg乙醇30 min后，灌胃三唑仑5 mg/kg，连续测定家兔大脑皮层和深部的心电图和脑电图，观察其行为学变化。给药后2.5小时，耳静脉血经气相色谱/质谱分析，三唑仑浓度为116 ng/ml。这个浓度太低，不会导致兔子死亡。可以想象，需要50倍的三唑仑浓度才能导致死亡。在上述条件下，给药三唑仑后约2小时出现持续性眼球震颤。我们还用Wistar雄性大鼠，在腹腔注射2g/kg乙醇后，测定了全脑多巴胺和5-羟色胺的浓度。与生理盐水治疗组相比，我们没有发现任何变化。而脑微透析法则能增加丘脑腹外侧核多巴胺和5-羟色胺的放电。推测在给予5 mg/kg三唑仑的情况下获得相同的结果。

项目成果

K. Yoshinoto. K. Uemare S. Konura et. al.: "The EHects of Newro to Nearo to tinsb ffyohro xydopnaine and 5.7-Oihydoythptamine in to the Rat Nacleus Accumbens on the Alcehsl Dhimking Beharior" Jpm. J. Legl Med.49(1). 11-19 (1995)

K·吉野登。

KOICHI UEMORA,SETSUO KOMORA: "Death caused by Thiazolam and Ethanal Intoxication" Am. J. Forensic Med. B Path. 16(1). 66-68 (1995)

KOICHI UEMORA、SETSUO KOMORA：“噻唑仑和乙醇中毒导致的死亡”Am。

Koichi Uemura and Setsuo Komura: "Death Caused by Triazolam and Ethanol Intoxication. Am J Forensic Med and Path" 16(1). 66-68 (1995)

Koichi Uemura 和 Setsuo Komura：“三唑仑和乙醇中毒导致的死亡。Am J Forensic Med and Path”16(1)。

KOICHI UEMURA,SETSUO KOMURA: "Death caused by Triazolam and Ethanol intoxication" Am.J.Forensic Med.& Path.16(1). 66-68 (1995)

KOICHI UEMURA、SETSUO KOMURA：“三唑仑和乙醇中毒导致的死亡”Am.J.Forensic Med。

K.Yoshimoto,K.Uemura,S.Komura et.al.: "The Effects of Neurotoxins 6-Hydroxydopamine and 5,7-Dihydroxytryptomine into the Rat Nuclens Accumbens on the Alcohol Drinkimg Behavior" Jpn.J.Legl.Med.49(1). 11-19 (1995)

K.Yoshimoto、K.Uemura、S.Komura 等人：“神经毒素 6-羟基多巴胺和 5,7-二羟基色胺进入大鼠伏核对饮酒行为的影响”Jpn.J.Legl.Med.49