A study on the evaluation of pre-treatment for forensic toxicological drug screening

法医毒理学药物筛查前处理评价研究

• 批准号: 06670474
• 负责人: KAGEURA Mitsuyoshi
• 金额: $ 0.77万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670474/
• 关键词: Forensic toxicology; Drug screening; Pre-treatment; Solid-phase extraction; Evaluation; Extrelut; Bond Elut; Clean Screen DAU; 薬毒物; スクリーニング

Different solid-phase extraction methods for the screenign of drugs prior to gas chromatography with nitrogen-phosphorus detection were compared The extractions including Bond Elut (Certify, CN,C18, C8), Chem Elut, Clean Screen DAU and Extrelut, were evaluated both by the absolute recoveries of 15 drugs from whole blood and by the degree of interferences from blank blood on the analysis of these drugs The mean absolute recoveries from the cartridge columns were 72.9,60.2,61.3,62.1,56.8,86.3, and 67.6%, with the coefficients of variation being 9.7,11.7,14.2,16.6,13.0,7.8, and 11.9%, respectively (the values of Bond Elut Certify and Clean Screen DAU are for 13 basiccompounds). The interferences from blank blood on the analysis of drugs were the smallest with Clean Screen DAU (basic extraction alone), and progressively greater on Bond Elut CN,Bond Elut Certify (basic extraction alone), Extrelut, Bond Elut C8, Bond Elut C18 and Chem Elut. The detection limits of the drugs in whole blood from Extrelut and Clean Screen DAU (basic drugs alone) were from 0.005 to 0.1 and from 0.005 to 0.02 mg/ml, respectively In conclusion, this study suggests that Clean Screeen DAU (for basic compounds) and Extrelut are the most suitable disposable cartridges for the routine screening of drugs in forensic toxicological analysis.

不同的固相萃取方法的screenign药物之前,气相色谱法与氮磷检测比较拔掉包括债券Elut(证明,CN、C18 C8),化学Elut,清洁屏幕道和Extrelut,进行评估通过15的绝对复苏药物从全血和血液从空白干扰的程度在分析这些药物的平均绝对复苏墨盒列分别为72.9,60.2,61.3,62.1,56.8,86.3,和67.6%，变异系数分别为9.7、11.7、14.2、16.6、13.0、7.8和11.9% （Bond Elut Certify和Clean Screen DAU分别为13种基本化合物）。空白血对药物分析的干扰在Clean Screen DAU（单纯基础萃取物）上最小，在Bond Elut CN、Bond Elut Certify（单纯基础萃取物）、Extrelut、Bond Elut C8、Bond Elut C18和Chem Elut上逐渐增大。exrelut全血药物检出限为0.005 ~ 0.1 mg/ml, Clean Screen DAU（单纯基础药物）检出限为0.005 ~ 0.02 mg/ml。综上所示，Clean Screen DAU（单纯基础药物）和exrelut是法医毒理学分析中常规药物筛查最合适的一次性药盒。