In vivo effects of sIL-IR and sIL-4R on IgE production and regulation of allergic reaction

sIL-IR和sIL-4R对IgE产生和过敏反应调节的体内影响

• 批准号: 06670498
• 负责人: MIYAJIMA Hiroaki
• 金额: $ 1.34万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670498/
• 关键词: sIL-1R; sIL-4R; IgE; GVHD; IgE; IL-1レセプター; IL-4レセプター; 可溶性IL-4リセプター; 可溶性IL-1リセプター; IgG1

The effect of sIL-4R and IL-1R were carried out on IgE production or murine GVHD models in vivo and in vitro.The effect of sIL-4R : IL-4 dependent cell line (CTLL-2) were cultured with sIL-4R and IL-4, CTLL-2 was inhibit. However, IgE production in mice infected with Nb. was not suppressive by the injection of sIL-4R in vivo.The effect of sIL-1R : Induction of GVH reaction in irradiated (BALB/c x C57BL/6)F1(CBF1) with bone marrow cells (BMC) plus spleen cells of (C57BL/6, or BALB/c) lead to GVHD (BMT/GVHD). The use of donor as BALB/c induced chonic GVHD whereas taht as C57BL/6 induced acute GVHD.When in murine acute GVHD model mice were treated with sIL-1R but sIL-4R, oranic falure of liver disease, splenomegaly and Immunodysfuncitons of T cell maturations were improved in GVHD mice.In our studies :1) sIL-4R were effective to suppression of IgE productions and inhibition of CTLL-2 cells.2) sIL-1R were effective to improvement of acute GVHD in mice.

本实验观察了sIL-4 R和IL-1 R对小鼠GVHD模型IgE产生的影响，sIL-4 R的影响：IL-4依赖性细胞系（CTLL-2）与sIL-4 R和IL-4共同培养，CTLL-2被抑制。然而，在Nb. sIL-1 R的作用：照射后的（BALB/c × C57 BL/6）F1代（CBF 1）小鼠骨髓细胞（BMC）加（C57 BL/6或BALB/c）脾细胞诱导GVH反应，导致GVHD（BMT/GVHD）。用BALB/c供体诱导慢性GVHD，用C57 BL/6供体诱导急性GVHD，用sIL-1 R治疗急性GVHD小鼠，可改善GVHD小鼠的肝功能损害、脾肿大和T细胞成熟的免疫功能障碍，本研究结果表明：1）sIL-4 R可抑制IgE的产生，抑制CTLL-2细胞的增殖; 2）sIL-1 R可改善急性GVHD小鼠。

项目成果

Ushiyama,C: "Anti-IL-4 antibody prevents a Graft-versus-Host disease (GVHD) in mice after bone marrow transplantation (BMT) : IgE allotype is an important marker of GVHD." J. Immunol.154. 2687-2696 (1995)

Ushiyama,C：“抗 IL-4 抗体可预防骨髓移植 (BMT) 后小鼠的移植物抗宿主病 (GVHD)：IgE 同种异型是 GVHD 的重要标志物。”

平野隆雄: "GVHDのマウスモデル" 血液腫瘍. 31. 327-333 (1995)

Takao Hirano：“GVHD 小鼠模型”血肿。31. 327-333 (1995)

Yamada, M: "Construction of a monoclonal antibody against Igh-7b." Immunogenetics. 40. 296-299 (1994)

Yamada, M：“构建抗 Igh-7b 的单克隆抗体。”

Ushiyama, C.: "Anti-IL-4 antibody prevents a Graft-versus-Host diseases (GVHD) in mice after bone marrow transplantation (BMT) : IgE allotype is an important marker of GVHD." J.Immunol. 154. 2687-2696 (1995)

Ushiyama, C.：“抗 IL-4 抗体可预防骨髓移植 (BMT) 后小鼠的移植物抗宿主病 (GVHD)：IgE 同种异型是 GVHD 的重要标志物。”

Yamada,M: "Construction of a monoclonal antibody against Ign-7b." Immunogenetics. 40. 296-299 (1994)

Yamada,M：“构建抗 Ign-7b 的单克隆抗体。”