Biomolecular analysis of liver carcinogenesis in hepatitis C virus infection.

丙型肝炎病毒（HCV）在晚期肝病中复制水平较高。HCVRNA阳性细胞常在晚期门脉周围、桥接和小叶内坏死的标本中检测到，但与炎性细胞浸润的程度无关。组织学活性指数显示，Fas抗原阳性细胞的门脉和门脉周围区域的炎症比Fas抗原阴性细胞更严重。HCV抗原阳性组Fas抗原阳性率高于HCV抗原阴性组。当通过RT-PCR检测Fas配体转录本的表达时，在肝脏浸润的单个核细胞和外周血单个核细胞中检测到扩增的信号，而在肝脏组织中仅检测到弱信号或根本没有检测到。这些结果表明，Fas配体-Fas系统可能在HCV感染肝细胞损伤中起重要作用。c-met mRNA在癌性病变中呈高表达，而在非癌性病变中无表达，且在未分化型肝癌中的表达率高于分化型肝癌。VPF/VEDFmRNA在癌细胞中过表达，提示其表达可能与肝内转移有关。

The replicative level of hepatitis C virus (HCV) is higher in advanced liver disease. HCV RNA-positive cells were frequently detected in specimens with advanced periportal, bridging and intralobular necrosis but showed no correlation with the extent of inflammatory cell infiltration. The histological activity index showed inflammation of both portal and periportal areas to be more severe in the Fas antigen-positive cells than the Fas antigen-negative ones. The prevalence of Fas antigen expression in the HCV antigen-positive group was higher than in the HCV antigen-negative group. When the expression of Fas ligand transcripts was tested by RT-PCR,the amplified signal was detected in liver-infiltrating mononuclear cells and peripheral blood mononuclear cells, whereas only a weak signal or none at all was detected in liver tissues. These results suggest that the Fas ligand-Fas system may play an important role in liver cell injury by HCV infection. The overexpression of c-met mRNA was found in cancerous lesion but not in non cancerous lesion, and its prevalence was higher in the undifferentiated type of hepatocellular carcinoma than in the differentiated type. Moreover, VPF/VEDF mRNA was overexpressed in cancer cells, suggesting that its expression may play a role in the intrahepatic metastasis.