STUDIES ON CLINICAL APPLICATION OF LASER PHOTODYNAMIC TREATMENT TO KERATOTIC SKIN DISEASES

以往对角化性皮肤病的移植嵌合体的功能形态进行了研究，并对这些移植嵌合体的增殖和分化进行了建模。这些移植的角化性皮肤病嵌合体也被用作激光光动力学(PDT)治疗角化性皮肤病的材料。本研究对激光光动力疗法进行了临床评价。在激光光敏治疗中，使用红宝石激光和酞菁和5-氨基乙酰丙酸作为光敏剂。研究结果如下：(1)寻常型银屑病皮损：分别采用皮肤移植(STM)、皮肤埋植移植(SETM)和皮肤开窗移植(SFTM)方法将寻常型银屑病皮损移植到裸鼠体内。一篇题为《通过开窗移植方法将寻常型银屑病皮损移植到裸鼠皮下组织中的超微结构》的论文目前正在《美国医学会杂志》(The Journ…)上发表。更多关于皮肤科的知识。SFTM移植的病变是进行激光光动力疗法的有用材料。激光光动力疗法治疗寻常型银屑病的临床应用。(2)角化性皮肤癌的发现：(1)以C_3H小鼠实验性皮肤癌为材料进行激光光敏治疗，结果已有报道，激光光疗后，真皮上部可见癌细胞固缩、水肿、出血。电子显微镜下，癌细胞胞核变形，线粒体脊消失。观察到激光PDT后线粒体的变形是最初的改变。(2)以皮肤癌和恶性黑色素瘤为临床资料。皮肤癌的研究以《裸鼠汗腺癌和激光光疗后癌细胞的超微结构》为题。激光PDT后癌细胞的表现以线粒体变形为初始改变。胞质内的细胞核和细胞器相继发生变化。真皮内可见水肿、出血。肿瘤肿块变性。使用移植嵌合体和体外材料对恶性黑色素瘤病变的调查仍在继续。(3)对Bowen病的病变进行了激光PDT治疗，结果表明激光PDT在治疗Bowen病的病变中有一定的临床应用价值。此外，在活性氧领域，对激光PDT作用机制的研究仍在继续。综上所述，激光PDT是一种有效的治疗角化性皮肤病的方法

Functional morphology was previously investigated in transplantation chimeras of keratotic skin diseases, and these transplantation chimeras underwent modeling on proliferation and differentiation. These transplantation chimeras of keratotic skin diseases were also used as materials for laser photodynamic treatment (PDT) of keratotic skin diseases. Clinical evaluation of laser PDT was perfomed by the present investigation. In laser PDT,ruby laser and phthalocyanine and 5-aminolevulinic acid as photosensitizer were used. The results are as follows ; (A) The findigs of psoriasis vulgaris : Psoriasis vulgaris lesions were transplanted into nude mice by the skin transplantation method (STM), skin embedding transplantation method (SETM), and skin fenestrating transplantation method (SFTM). A paper entitled "Ultrastructure of the lesions of psoriasis vulgaris transplanted into the subcutaneous tissue of nude mice, by the fenestrating transplantation method" is currently in press in the Journ … More al of Dermatology. Lesions transplnted by SFTM were useful materials for laser PDT was investigated. Clinical application of laser PDT on psoriasis vulgaris lesions. (B) The findings of keratotic skin cancer : (1) Experimental skin cancer in C_3H mice was used as materials for laser PDT.The results have previously been reported, and after laser PDT,pyknotic cancer cells, edema and bleeding were revealed in the upper dermal portion. Electron-microscopically, nuclei of cancer cells were deformed and mitochondrial cristae disappeared. It was observed that the mitochondrial deformation was the initial change after laser PDT.(2) Skin cancer and malignant melanoma were used as clinical materials. Investigation of skin cancer was reported under the title "Ultrastructure of sweat organ cancer in nude mice and cancer cells after laser PDT". The findings of cancer cells after laser PDT showed mitochondrial deformation as the initial changes. Nuclei and organelles changed successively in the cytoplasm. In the dermis, edema and bleeding were observed. The tumor masses degenerated. Investigation of malignant melanoma lesions using transplantation chimeras and in vitro-materials is continuing. (3) In the lesions of Bowen's disease, the results of laser PDT were reported, and findings revealed that clinical application of laser PDT was useful in treating lesions of Bowen disease. Furthermore, the investigation of the action mechanisms of laser PDT are continuing in the field of active oxygen.In summary, it was concluded that laser PDT was a useful treatment for the keratotic skin diseases Less