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STUDIES ON CLINICAL APPLICATION OF LASER PHOTODYNAMIC TREATMENT TO KERATOTIC SKIN DISEASES

激光光动力治疗角化性皮肤病的临床应用研究

基本信息

批准号：
06670857
负责人：
UEDA Keiichi
金额：
$ 0.9万
依托单位：
FUKUI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Functional morphology was previously investigated in transplantation chimeras of keratotic skin diseases, and these transplantation chimeras underwent modeling on proliferation and differentiation. These transplantation chimeras of keratotic skin diseases were also used as materials for laser photodynamic treatment (PDT) of keratotic skin diseases. Clinical evaluation of laser PDT was perfomed by the present investigation. In laser PDT,ruby laser and phthalocyanine and 5-aminolevulinic acid as photosensitizer were used. The results are as follows ; (A) The findigs of psoriasis vulgaris : Psoriasis vulgaris lesions were transplanted into nude mice by the skin transplantation method (STM), skin embedding transplantation method (SETM), and skin fenestrating transplantation method (SFTM). A paper entitled "Ultrastructure of the lesions of psoriasis vulgaris transplanted into the subcutaneous tissue of nude mice, by the fenestrating transplantation method" is currently in press in the Journ … More al of Dermatology. Lesions transplnted by SFTM were useful materials for laser PDT was investigated. Clinical application of laser PDT on psoriasis vulgaris lesions. (B) The findings of keratotic skin cancer : (1) Experimental skin cancer in C_3H mice was used as materials for laser PDT.The results have previously been reported, and after laser PDT,pyknotic cancer cells, edema and bleeding were revealed in the upper dermal portion. Electron-microscopically, nuclei of cancer cells were deformed and mitochondrial cristae disappeared. It was observed that the mitochondrial deformation was the initial change after laser PDT.(2) Skin cancer and malignant melanoma were used as clinical materials. Investigation of skin cancer was reported under the title "Ultrastructure of sweat organ cancer in nude mice and cancer cells after laser PDT". The findings of cancer cells after laser PDT showed mitochondrial deformation as the initial changes. Nuclei and organelles changed successively in the cytoplasm. In the dermis, edema and bleeding were observed. The tumor masses degenerated. Investigation of malignant melanoma lesions using transplantation chimeras and in vitro-materials is continuing. (3) In the lesions of Bowen's disease, the results of laser PDT were reported, and findings revealed that clinical application of laser PDT was useful in treating lesions of Bowen disease. Furthermore, the investigation of the action mechanisms of laser PDT are continuing in the field of active oxygen.In summary, it was concluded that laser PDT was a useful treatment for the keratotic skin diseases Less

先前对角化性皮肤病的移植嵌合体的功能形态进行了研究，并对这些移植嵌合体进行了增殖和分化建模。这些角化性皮肤病的移植嵌合体也被用作角化性皮肤病的激光光动力治疗（PDT）的材料。本研究对激光PDT进行了临床评价。在激光PDT中，采用红宝石激光以及酞菁和5-氨基乙酰丙酸作为光敏剂。结果如下； (A)寻常型银屑病的研究：采用皮肤移植法(STM)、皮肤包埋移植法(SETM)、皮肤开窗移植法(SFTM)将寻常型银屑病皮损移植到裸鼠体内。题为“通过开窗移植法将寻常型银屑病病变移植到裸鼠皮下组织中的超微结构”的论文目前正在《皮肤病学杂志》上发表。 SFTM 移植的病变是用于激光 PDT 的有用材料。激光PDT治疗寻常型银屑病皮损的临床应用(B) 角化性皮肤癌的发现： (1) 以C_3H小鼠实验性皮肤癌为材料进行激光PDT。该结果先前已报道，激光PDT后，可见上真皮部分癌细胞固缩、水肿和出血。电子显微镜下，癌细胞的细胞核变形，线粒体嵴消失。观察到线粒体变形是激光PDT后最初的改变。(2)以皮肤癌和恶性黑色素瘤为临床材料。皮肤癌的研究以“裸鼠汗液器官癌的超微结构和激光PDT后的癌细胞”为题进行了报道。激光PDT后癌细胞的发现显示线粒体变形是最初的变化。细胞质中的细胞核和细胞器相继发生变化。在真皮中观察到水肿和出血。肿瘤块变性。使用移植嵌合体和体外材料对恶性黑色素瘤病变的研究仍在继续。 (3)在Bowen病病变中，报道了激光PDT的结果，发现临床应用激光PDT对于治疗Bowen病病变有一定作用。此外，活性氧领域对激光PDT作用机制的研究仍在继续。综上所述，激光PDT对于角化性皮肤病是一种有效的治疗方法。