LOCALIZATION OF CORNIFIN IN EPIDERMAL KERATINOCYTE AND ITS EXPRESSION IN SKIN DISEASES

本研究通过Northern blot杂交、免疫blot分析和免疫组织化学比较了cornifin α和β在几种组织中的表达。通过Northern blot杂交，在正常表皮、银屑病表皮、皮肤鳞状细胞癌和口腔牙龈上皮中检测到0.8 kb的cornifinalpha转录本。1.1 kb的cornifin β转录本仅在口腔牙龈上皮和皮肤疣状癌中检测到，而在正常表皮和银屑病表皮以及皮肤鳞状细胞癌中均未检测到。免疫印迹分析显示，人角蛋白α /SPRR1的SQ37C-Ab在分化的正常人表皮角质形成细胞、头皮和足底正常表皮以及银屑病表皮提取物中识别14.0 kd蛋白。该抗体识别正常牙龈上皮提取液中的14.0、16.0和26.0 kD蛋白，皮肤疣状癌提取液中的14.0、16.0、25.0 kD蛋白和一些表观分子量小于14.0 kD的蛋白。针对cornifin β的cl12pepb - ab与表皮组织提取物不发生反应，但强烈识别牙龈上皮组织提取物中26.0 kD的蛋白。对正常皮肤和各种皮肤病标本的分析表明：1)角质蛋白α在表皮的颗粒层、毛囊的内根鞘以及口腔和食管上皮的上鳞状层表达；2)在寻常型银屑病的增生性、粒细胞性病变和扁平苔藓的高粒性病变中，角质蛋白α均表达上调。3)除疣状癌外，cornifin β在正常口腔和食管上皮中表达，在皮肤和皮肤病中不表达。

In this study, the expression of cornifin alpha and beta was compared in several tissues by Northern blot hybridization, immunoblot analyzes, and immunohistochemstry. By Northern blot hybridization, an 0.8 kb cornifinalpha transcript was detected in normal epidermis, psoriatic epidermis, squamous cell carcinoma of the skin, and oral gingival epithelia. The 1.1 kb cornifin beta transcript was detected only in oral gingival epithelia and verrucous carcinoma of the skin, but neither in normal and psoriatic epidermis nor in squamous cell carcinoma of the skin. Immunoblot analysis revealed that SQ37C-Ab for human cornifin alpha/SPRR1, recognized a 14.0-kD protein in extracts from differentiated normal human epidermal keratinocytes, normal epidermis from scalp and sole, and psoriatic epidermis. The antibody recognized 14.0,16.0, and 26.0 kD proteins in extracts from normal gingival epithelia, and 14.0,16.0,25.0 kD proteins, and some proteins with apparent molecular weight smaller than 14.0 kD in extracts from verrucous carcinoma of the skin. The CL 12PEPB-Ab for cornifin beta did not react with extracts from epidermal tissues but strongly recognized a 26.0 kD protein in extracts from gingival epithelial tissues. Analyzes of specimens from normal skin and various skin diseases showed that 1) cornifin alpha was expressed in the granular layr of the epidermis, in the inner root sheath of the hair follicle, and in the upper squamous layrs of oral and esophageal epithelia, 2) cornifin alpha was up-regulated both in hyperplastic, agranulocytic lesions as observed in psoriasis vulgaris and in hypergranulotic lesions as observed in lichen planus, 3) cornifin beta was expressed in normal oral and esophageal epithelia but not in skin and skin diseases, except for verrucous carcinoma.