Molecular biology of morphological preservation of radiotherapy

为了探讨放射治疗(RT)的形态保存机制，对放射治疗的临床、病理和分子生物学进行了研究。通过对放疗前和放疗后的形态进行摄影和放射学比较，得出结论：形态保存不仅仅是有序的组织修复，而是复杂的再生。对形态发育中重要的细胞-细胞黏附分子E-钙粘附素的免疫组化染色显示，65Gy射线照射喉面5年后，E-钙粘附素仍呈阳性表达。细胞迁移实验显示，照射10GyMRC5条件培养液迁移的HSC3和CA9-22细胞数明显多于未照射的MRC5。免疫组织化学定量分析显示，照射10GyMRC5条件培养液中HGF的含量高于未照射的MRC5。以上结果提示，放疗后组织形态的保存有赖于对正常组织的粘膜调控，其机制还有待进一步研究。

To investigate the mechanism of morphological preservation of radiation therapy (RT), clinical, pathological, and molecular biological research was undertaken. By photographical and radilogical comparison between the pre-RT morphology and post-RT morphology, it was conclusive that morphological preservation is not merely ordinal tissue repair, but rather complex regeneration. Immunostaning of E-cadherin, which is one of the cell-cell adhesion molecule important in morphological developmement, showed that E-cadherin is stained even 5 years after the 65 Gy irradiation to the larynxgeal surface. Cell migration assay showed that more number of epitherial cells, HSC3 and CA9-22, were migrated by the conditioned medium of MRC5 which was irradiated 10 Gy than that of non-irradiated MRC5 Immunobiochemical quantitative analysis showed that the conditioned medium of MRC5 has more amount of HGF after 10 Gy irradiation than that of non-irradiated MRC5. These resultus suggested that morphological preservation after radiotherapy is depend on the molucular control of normal tissue, of which mechanism still await more investigation.