Effects of stem cell factor and extracellular matrix on melanocyte differentiation in mouse neural crest cell culture.

干细胞因子和细胞外基质对小鼠神经嵴细胞培养中黑素细胞分化的影响。

• 批准号: 06670885
• 负责人: KAWA Yoko
• 金额: $ 1.41万
• 依托单位: St.Marianna University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670885/
• 关键词: stem cell factor; c-Kit; neural crest; melanogenesis; melanocyte-differentiation; tyrosinase; TRP1; TRP2; c-Kit; TPR2

We investigated the role of soluble stem cell factor (s-SCF) in the melanocyte development of cultured mouse neural crest cells (NCC) by using immunohistochemical techniques and DOPA reaction. c-KIT (+) dendritic cells were found on Day 3 of the explant culture with continuous presence of s-SCF (50ng/ml) and reached a plateau after Day 9, while there were no positive cells left at Day 9 without s-SCF.When s-SCF was added on Day 5 or after, c-KIT (+) cells did not appear. Tyrosinase, TRP1, and TRP2 positive cells all appeared by Day 9 in cultured NCC with s-SCF.These results suggest that SCF may play a role in the development of c-KiIT (+) cells and that there is a critical time (Days 0-5) when s-SCF must be added to induce c-KIT (+) cells DOPA positive melanocytes but no mature melanocytes were found in the explant culture with s-SCF,while the effects of s-SCF on both DOPA positive melanocyte appearance and the differentiation into mature melanocytes were markedly enhanced by the addition of cholera toxin (CT) to the culture. It is indicated that additional factors, such as CT,may be necessary to induce the differentiation into mature melanocytes.

应用免疫组织化学技术和多巴反应研究了可溶性干细胞因子（s-SCF）在体外培养的小鼠神经嵴细胞（NCC）黑素细胞发育中的作用。c-KIT（+）树突状细胞在s-SCF（50 ng/ml）作用下培养第3天出现，第9天后达平台期，而在无s-SCF作用下培养第9天，c-KIT（+）树突状细胞消失，第5天及以后加入s-SCF后，c-KIT（+）树突状细胞消失。用s-SCF培养的NCC在第9天均出现酪氨酸酶、TRP 1和TRP 2阳性细胞。这些结果表明SCF可能在c-KiIT（+）细胞的发育中发挥作用，并且存在一个关键时间（第0-5天）当必须加入s-SCF以诱导c-KIT（+）细胞时，在含有s-SCF的外植体培养物中没有发现DOPA阳性黑素细胞，而加入霍乱毒素（CT）后，s-SCF对DOPA阳性黑素细胞的出现和向成熟黑素细胞分化的作用均显著增强。这表明，额外的因素，如CT，可能是必要的，以诱导分化为成熟的黑素细胞。

项目成果

河陽子: "マウス神経冠培養系でのメラノサイトの分化におけるstem cell factorの役割" 日本皮膚科学会雑誌. (発表予定).

Yoko Kawa：“干细胞因子在小鼠神经嵴培养系统中黑素细胞分化中的作用”，日本皮肤病学会杂志（待出版）。