Effects of CSF from schisophrenic patients on growth and differentiation of neurobl a stoma cells

精神分裂症患者脑脊液对神经母细胞生长和分化的影响

• 批准号: 06670953
• 负责人: SHIRASAWA Hiroshi
• 金额: $ 1.34万
• 依托单位: Chiba university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670953/
• 关键词: schizophrenia; neural cell; differentiation; 神経芽腫細胞

Effects of CSF from schizophrenic patients on a neuroblastoma cell line RT-BM-1 were studied. The CSFs taken from two informed-consent schizophrenic patients were added to the culture medium. The growth rate, population doubling time and saturation density of RT-BM-1 Cultared in the CSF-added medium were sameas those of control cells. Effects of CSFs from schizophremic patients on the differentiation of RT-BM-1 cells by retinoic acid were also examined. However, no differences were observed in the morphological changes induced by the retinoic acid as well as the amount of MAP 2 demonstrated by western blotting.To investigate further the etiology of the schizophrenia, we attempted to clone cDNAs of genes differentially expressed by adminstration of Phencylidine (PCP) . We have identified several unknown and known genes differentially expressed genes in brains of PCP-administrated rats. We also cloned the cDNA of the mouse mxil gene (E.Shimizu et al., 1995) , and found that the expression of the mxil elevates during neuronal differntiation (E.Shimizu et al., in press).

研究了精神分裂症患者脑脊液对神经母细胞瘤细胞系RT-BM-1的作用。将两名知情同意的精神分裂症患者的CSF加入培养基中。RT-BM-1在添加CSF的培养基中的生长速度、群体倍增时间和饱和密度与对照细胞相同。还检查了精神分裂症患者的CSF对维甲酸诱导RT-BM-1细胞分化的影响。为进一步研究精神分裂症的发病机制，我们克隆了苯环苯胺（Phencylidine，PCP）诱导的差异表达基因的cDNA。我们已经确定了几个未知的和已知的基因差异表达的基因在PCP给药大鼠的大脑。我们还克隆了小鼠mxil基因的cDNA（E.Shimizu等人，1995），并发现mxil的表达在神经元分化期间升高（E.Shimizu等，印刷中）。

项目成果

H.Shirasawa et al.: "Transcription-modulatory activity of full-length E6 and E6^*I proteins of human papillomavirus type 16" Virology. 203. 36-42 (1994)

H.Shirasawa 等人：“人乳头瘤病毒 16 型全长 E6 和 E6^*I 蛋白的转录调节活性”病毒学。

T.Matsunaga et al.: "Expression of neuronal src mRNA as a favorable marker and inverse correlation to N-myc gene amplification in human neuroblastomas" Int.J.Cancer. 58. 793-798 (1994)

T.Matsunaga 等人：“神经元 src mRNA 的表达作为有利标记，与人神经母细胞瘤中 N-myc 基因扩增呈负相关”Int.J.Cancer。

K.Shimizu et al.: "Alternative splicing of human papillomavirus type-16 E6 mRNA in mouse and monkey cell lines" Int.J.Oncology. 4. 971-976 (1994)

K.Shimizu 等人：“小鼠和猴细胞系中人乳头瘤病毒 16 型 E6 mRNA 的选择性剪接”Int.J.Oncology。

Shimizu,E.et al.: "Cloning and sequencing of the murine Mxi1 cDNA" Gene. 152. 283-284 (1995)

Shimizu,E.et al.：“小鼠 Mxi1 cDNA 的克隆和测序”基因。

Matsunaga,T.et al.: "Expression of neuronal src mRNA as a favorable marker and invorse correlation to N‐myc gene amplification in human neuroblastomas" International Journal of Cancer. 58. 793-798 (1994)

Matsunaga, T. 等人：“神经元 src mRNA 的表达作为有利标记，与人类神经母细胞瘤中 N-myc 基因扩增呈负相关”，《国际癌症杂志》58. 793–798 (1994)。