Effects of CSF from schisophrenic patients on growth and differentiation of neurobl a stoma cells

研究了精神分裂症患者脑脊液对神经母细胞瘤细胞系RT-BM-1的作用。将两名知情同意的精神分裂症患者的CSF加入培养基中。RT-BM-1在添加CSF的培养基中的生长速度、群体倍增时间和饱和密度与对照细胞相同。还检测了来自癫痫患者的CSF对维甲酸诱导的RT-BM-1细胞分化的影响。为进一步研究精神分裂症的发病机制，我们克隆了苯环苯胺（Phencylidine，PCP）诱导的差异表达基因的cDNA。我们已经确定了几个未知的和已知的基因差异表达的基因在PCP给药大鼠的大脑。我们还克隆了小鼠mxil基因的cDNA（E.Shimizu等人，1995），并发现mxil的表达在神经元分化期间升高（E.Shimizu等，出版中）。

Effects of CSF from schizophrenic patients on a neuroblastoma cell line RT-BM-1 were studied. The CSFs taken from two informed-consent schizophrenic patients were added to the culture medium. The growth rate, population doubling time and saturation density of RT-BM-1 Cultared in the CSF-added medium were sameas those of control cells. Effects of CSFs from schizophremic patients on the differentiation of RT-BM-1 cells by retinoic acid were also examined. However, no differences were observed in the morphological changes induced by the retinoic acid as well as the amount of MAP 2 demonstrated by western blotting.To investigate further the etiology of the schizophrenia, we attempted to clone cDNAs of genes differentially expressed by adminstration of Phencylidine (PCP) . We have identified several unknown and known genes differentially expressed genes in brains of PCP-administrated rats. We also cloned the cDNA of the mouse mxil gene (E.Shimizu et al., 1995) , and found that the expression of the mxil elevates during neuronal differntiation (E.Shimizu et al., in press).